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白僵菌纯孢子粉与稀释剂按一定比例混合,配成所需浓度,用于超低容量防治森林害虫,杀虫效果比常规喷粉高,成本低,是我省近几年推广应用防治松毛虫的主要手段。目前我省主要采用固体发酵机械分离的方法生产孢子粉,含孢量为1,200亿/g 左右,也曾用过平板培养生产白僵菌孢子粉,含孢量为2,200亿/g 左右。为了提高白僵菌孢子粉的纯度、产量和节约原材料,我们拟在原平板培养的基础上,开展平板机械化生产白僵菌孢子粉的研究。本文就着重介绍采用多因子正交试验法筛选较适合的接种菌龄、液体培养基的配方、平板载体厚度、平板载体培养基配方和单位面积接种量。因子与水平影响平板培养白僵菌孢子的产量和成本,除了采用优良的白僵菌菌种和控制最适温湿度外,还有与发酵的菌龄、液体培养基的配方,平板载体厚度、平板载体培养基的配方和单位面积接种量等五个因子有关,为
Beauveria bassiana pure spore powder and diluent according to a certain percentage of mixing, dubbed the desired concentration for the ultra-low capacity of forest pests, insecticidal effect than the conventional high-powder, low cost, is the promotion and application of prevention and treatment in our province in recent years The main means of pine caterpillars. At present, our province mainly adopts the method of solid fermentation machinery to produce spore powder, the spore content is about 120 billion / g, and the slab powder of Beauveria bassiana has also been used in slab culture, the spore content is about 220 billion / g. In order to improve the purity and yield of Beauveria bassiana spore meal and to save the raw materials, we intend to carry out the mechanization of the production of Beauveria bassiana spore powder on the basis of the original plate culture. This article focuses on the use of multi-factor orthogonal test screening more suitable inoculation age, liquid medium formulation, plate carrier thickness, plate carrier medium formula and inoculum size per unit area. Factors and levels affect Beauveria bassiana spore yield and cost in plate culture, in addition to the use of excellent Beauveria bassiana strains and control of the optimum temperature and humidity, but also with the fermentation of bacteria age, liquid medium formulation, plate carrier thickness, Tablet carrier medium formula and inoculum per unit area of five factors such as