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本文运用MTT实验考察姜黄素和右旋龙脑分别在单独作用和联合作用时对黑色素瘤细胞A375存活率的影响,并进一步运用流式细胞术分析右旋龙脑联合姜黄素抑制黑色素瘤细胞A375增殖的原因。结果表明,姜黄素单独作用对A375细胞增殖有显著的抑制效应,而右旋龙脑单独作用对A375细胞增殖没有表现出明显的抑制作用。当用40μg/m L右旋龙脑预处理3 h,再与20μmol/L姜黄素联合作用A375细胞72 h后,其细胞存活率达到最低为10.93%,这比20μmol/L姜黄素单独处理时细胞的存活率降低了18.27%。进一步的流式结果表明,相比单独姜黄素处理,右旋龙脑与姜黄素联合处理后能明显提高Sub G1峰和G2/M比例,右旋龙脑(40μg/m L)与姜黄素(20μmol/L)联合作用黑色素瘤细胞A375后,其Sub G1和G2/M的含量分别从对照组的2.0%和16.4%升高到16.8%和40.1%,表明右旋龙脑与姜黄素主要是通过诱导细胞凋亡和G2/M阻滞来抑制黑色素瘤细胞A375增殖。
In this paper, MTT assay was used to investigate the effect of curcumin and dextro-rotundine on the survival rate of A375 melanoma cells separately and in combination with flow cytometry analysis of dexamethasone and curcumin inhibition of melanoma A375 Reasons for proliferation. The results showed that curcumin alone had a significant inhibitory effect on the proliferation of A375 cells, while dextran alone did not significantly inhibit the proliferation of A375 cells. The cell survival rate of A375 cells reached 10.93% when pretreated with 40μg / mL dextromethorphan for 3 h and then treated with 20μmol / L curcumin for 72 h, which was lower than that of 20μmol / L curcumin alone Cell viability decreased by 18.27%. Further flow cytometry results showed that the combination of dextran and curcumin significantly increased the ratio of Sub G1 peak to G2 / M, compared with curcumin (40 μg / m L) 20μmol / L) combined with melanoma A375, the contents of Sub G1 and G2 / M increased from 2.0% and 16.4% in control group to 16.8% and 40.1% respectively, indicating that dextran and curcumin are mainly Inhibition of melanoma A375 proliferation by induction of apoptosis and G2 / M arrest.