本文报告了在25±0.5℃、75±5%的相对湿度条件下,应用长筒式倒置喂血装置和Parafilm膜-猪血在实验室内离体养殖方形黄鼠蚤松江亚种获得成功,并已连续繁殖50代。规范化养殖8代。各项技术数据为:结茧率58.8%,羽化率93.8%,成长率55.8％,繁殖力0.85只蚤/·日。饥饿成蚤寿命为18.2±1.6天,17.7±1.6天;体更0.43±0.03mg,0.34±0.02mg。采用离体养殖的方形黄鼠蚤与鼠体养殖相比无明显差异,完全可以取代鼠体养殖。 In this paper, we report the success of in vitro cultivation of T. gonorrhoeae Songjiang subspecies in a laboratory using a long-barrel inverted blood donor and Parafilm-porcine blood at 25 ± 0.5 ℃ and 75 ± 5% relative humidity. And has been breeding for 50 generations. Standardized breeding 8 generations. The technical data are as follows: cocooning rate 58.8%, emergence rate 93.8%, growth rate 55.8%, fecundity 0.85 fleas / day. The hunger flea life was 18.2 ± 1.6 days, 17.7 ± 1.6 days; the body was 0.43 ± 0.03 mg, 0.34 ± 0.02 mg. There was no significant difference between the cultured carp and the cultured rat body, which can completely replace the mouse body culture.