论文部分内容阅读
目的探讨G蛋白偶联受体30(GPR30)m RNA和蛋白在临产前、后子宫平滑肌组织中的表达及其与分娩发动的相关性。方法 2011年3月至2012年3月在青岛市市立医院选取临产前进行选择性剖宫产的足月妊娠妇女作为未临产组(n=40),自然临产进入产程活跃期后进行急症剖宫产的足月妊娠妇女作为临产组(n=50),于剖宫产术中取少量子宫平滑肌组织,采用实时荧光定量PCR技术和Western blot技术检测两组子宫平滑肌组织中GPR30 m RNA和蛋白的表达量;并于术前取静脉血,采用放射免疫法测定血清中雌二醇(E2)、雌三醇(E3)以及孕酮(P)的含量。结果实时荧光定量PCR方法检测临产组和未临产组子宫平滑肌组织中均有GPR30 m RNA表达,临产组样本△Ct为3.08±0.35,未临产组样本△Ct为4.02±0.67,差异具有统计学意义(P<0.05),用双△Ct法进行相对定量分析,临产组GPR30 m RNA表达量是未临产组的1.92倍。Western blot技术检测临产组和未临产组子宫平滑肌组织中均有GPR30蛋白表达,临产组GPR30蛋白相对表达量4.06±0.25明显高于未临产组1.94±0.23,差异有统计学意义(P<0.05)。临产组血清E2、E3浓度[(7882.41±921.76)pmol/L,(544.12±15.58)nmol/L]均显著高于未临产组[(5210.72±873.13)pmol/L,(326.36±12.26)nmol/L]。结论足月妊娠分娩发动时GPR30高表达可能参与介导雌激素对子宫收缩的调节作用。
Objective To investigate the expression of G protein - coupled receptor 30 (GPR30) m RNA and protein in uterine smooth muscle before and after prenatal and postnatal period and its relationship with labor onset. Methods From March 2011 to March 2012, full-term pregnant women who had elective cesarean section before labor were selected as the non-labor group (n = 40) in Qingdao Municipal Hospital. After spontaneous labor, they entered the active phase of labor and performed emergency cesarean section The uterine smooth muscle tissue of cesarean section was taken as the labor group (n = 50). The real-time quantitative PCR and Western blot were used to detect the GPR30 mRNA and protein in uterine smooth muscle of the two groups The blood samples were collected before operation and the contents of estradiol (E2), estriol (E3) and progesterone (P) in serum were determined by radioimmunoassay. Results Real-time fluorescence quantitative PCR was used to detect the expression of GPR30 mRNA in uterine smooth muscle of both untreated and untreated groups. The △ Ct of the sample in the labor group was 3.08 ± 0.35 and the △ Ct in the non-patient group was 4.02 ± 0.67, the difference was statistically significant (P <0.05). Relative quantitative analysis by double △ Ct method showed that GPR30 mRNA expression in labor group was 1.92 times of that in non-labor group. The expression of GPR30 protein in labor group and non-labor group was significantly higher than that in non-labor group (P <0.05). The relative expression of GPR30 protein in labor group was 4.06 ± 0.25, significantly higher than that in non-labor group (1.94 ± 0.23, P <0.05) . The concentrations of E2 and E3 in labor group were significantly higher than those in non-labor group [(5210.72 ± 873.13) pmol / L, (326.36 ± 12.26) nmol / L, L]. Conclusion The high expression of GPR30 may affect the regulatory effect of estrogen on uterine contractions during full-term pregnancy delivery.