将人胰岛素原类似物(BKRA)基因通过人工接头串联,形成基因三联体,接头部分的氨基酸序列为RRNSM.该串联基因与表达载体PET?28A(+)重组后,实现了在大肠杆菌中的高效融合表达,表达产物以包涵体形式存在,约占细菌总蛋白的31％.以HITRAP凝胶进行亲和层析初步纯化,纯度可达93％.放射免疫测定表明,纯化的融合蛋白具有胰岛素抗原活性,为进一步生产基因工程胰岛素打下了基础.“,”The human proinsulin analog(BKRA)gene molecules were linked tandemly with synthetic nucleotide linker coding RRNSM to form a triplet molecule.The BKRA gene triplet was cloned into pET 28a(+),an expression plasmid,and expressed in E.coli in a fusion protein fashion.The exressed proteins aggregated into inclusion body,and weigh about 31%of the total proteins of the total proteins of the expression bacteria.The expressed proteins are easily purified by HiTrap affinity matrix up to putity of 93%.Human ins...