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用激素治疗转移的乳腺癌只有1/3有效,所以有必要预测该肿瘤是否是依赖激素类型的。无论测定组织中的激素受体,还是用放射免疫方法测定乳汁或血清中的乳蛋白,结果都不理想。其原因除了免疫学方法上的技术问题之外,可能是由于检测的是激素作用的最终产物——蛋白质,而不是转录的最初产物——mRNA。因此较好的途径似乎是检测正常和乳腺癌组织转录活性而不是翻译活性的差异。本文叙述了正常人乳腺组织由含PolyA-的mRNA的分离,然后根据在无细胞体系中mRNA指导合成的酪蛋白和α-乳清蛋白来证实存在的mRNA种类。用同样方法,证实了5例乳癌患者中有两例存在着指导α-乳清蛋白的mRNA。
Only 1/3 of the cancers metastasized with hormones are effective, so it is necessary to predict if the tumor is hormone-dependent. Both the determination of hormone receptors in tissues and the determination of milk proteins in milk or serum by radioimmunoassay showed unsatisfactory results. The reason for this may be due to the technical problems with immunological methods, possibly due to the detection of the end product of the hormonal effect, the protein, rather than the original product of transcription, mRNA. Thus, a better approach seems to be to detect differences in normal and breast cancer tissue transcriptional activity rather than translational activity. This article describes the isolation of normal human mammary gland tissue from PolyA-containing mRNAs, and then confirms the presence of mRNA species based on mRNA-guided synthesis of casein and alpha-lactalbumin in a cell-free system. In the same way, it was confirmed that mRNA for α-lactalbumin was present in two of five breast cancer patients.