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该研究运用生物分子网络分析方法,预测注射用丹参多酚酸盐与阿司匹林联合用药治疗稳定型心绞痛的作用机制。从Genecards,STITCH,Dis Ge NET数据库获取注射用丹参多酚酸盐、阿司匹林及稳定型心绞痛(stable angina pectoris,SAP)的相关基因;采用Agilent Literature Search文献搜索软件构建生物分子网络;经AP,MCODE和MCL 3种方法对生物网络进行模块识别;通过DAVID软件进行相关KEGG通路识别。结果表明注射用丹参多酚酸盐和阿司匹林对SAP分子网络的覆盖率分别为45.92%,62.56%,联合用药的覆盖率为71.64%。注射用丹参多酚酸盐、阿司匹林与SAP前10个重要节点中,MAPK14,MAPK8,IL-6,IL-8为2个药物共同重叠的SAP重要节点,AKT1和IFNG为注射用丹参多酚酸盐单独重叠的SAP重要节点,EPHB2和TP53为阿司匹林单独重叠的SAP重要节点。2个药物共同参与的SAP相关信号通路包括JAK-STAT信号通路和MAPK信号通路等;注射用丹参多酚酸盐单独参与的SAP相关信号通路包括VEGF信号通路和1型糖尿病信号通路;阿司匹林单独参与的SAP相关信号通路包括AA代谢、亚油酸代谢信号通路等。该研究表明联合用药一方面在抗炎症反应和抑制动脉粥样硬化发展方面有疗效增强作用;另一方面注射用丹参多酚酸盐在阿司匹林抗血小板聚集的基础上能够保护血管内细胞和调节糖代谢,起到疗效相加作用。
This study used biomolecular network analysis to predict the mechanism of action of Salviae miltiorrhizae in combination with aspirin in the treatment of stable angina pectoris. Genes of salvia miltiorrhiza polyphenols, aspirin and stable angina pectoris (SAP) were obtained from Genecards, STITCH and Dis Ge NET databases. Biomolecular networks were constructed using Agilent Literature Search search software. After AP, MCODE And MCL three kinds of methods to identify the biological network module; through DAVID software related KEGG pathway identification. The results showed that the coverage of SAP molecule network with Salviae miltiorrhizae polyphenols and aspirin were 45.92% and 62.56% respectively, and the coverage of combined drug was 71.64%. MAPK14, MAPK8, IL-6 and IL-8 were the important nodes of SAP in which two drugs overlap together, and AKT1 and IFNG were the main components of Salviae miltiorrhizae for injection Salt alone overlap SAP important node, EPHB2 and TP53 aspirin overlapping SAP important node. SAP-related signaling pathways involved in the two drugs include JAK-STAT signaling pathway and MAPK signaling pathway; the SAP-related signaling pathways involved in Salviae miltiorrhizae injection alone include the VEGF signaling pathway and the type 1 diabetes signaling pathway; and aspirin alone participates The SAP-related signaling pathways include AA metabolism, linoleic acid metabolic signaling pathways and the like. The study shows that combination therapy on the one hand in the anti-inflammatory response and inhibit the development of atherosclerosis have a therapeutic effect; the other hand, injection of salvianolic acid polyphenols in aspirin anti-platelet aggregation based on the protection of vascular cells and regulate sugar Metabolism, play a role in the additive effect.