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利用反相高效液相色谱法快速测定各种复合甜味剂中Ａｓｐａｒｔａｍ，用ＯＤＳ短柱（ＵｌｔｒａｓｐｈｅｒｅＸＬ－ＯＤＳ，３μｍ，４．６ｍｍｉ．ｄ．×７．０ｃｍ），以ＣＨ３ＯＨ－０．０２ｍｏｌ／ＬＮＨ４Ａｃ为流动相进行分离，紫外检测器２２０ｎｍ检测，根据色谱保留时间结合对组分不停泵紫外光谱扫描进行定性，用外标法峰高定量，方法最小检出浓度为５μｇ／Ｌ，标准曲线在４０～２００ｍｇ／Ｌ浓度范围内呈线性，相关系数在０．９９９以上，平均添加回收率为９２％，其它复合组分不干扰测定。方法简便、快速、灵敏，非常适合于日常监督检测。
Aspartam in various complex sweeteners was rapidly assayed by reversed-phase high-performance liquid chromatography with an ODS short column (Ultrasphere XL-ODS, 3 μm, 4.6 mm i.d. × 7.0 cm), CH3OH-0.02 mol / L NH4Ac The mobile phase was separated and detected by UV detector at 220nm. According to the chromatographic retention time, UV-Vis spectra of the components were qualitatively and qualitatively determined by external standard peak method. The minimum detectable concentration was 5μg / L. The standard curve was 40 ~ 200mg / L concentration range was linear, the correlation coefficient of 0.999 or more, the average recovery rate was 92%, other composite components do not interfere with the determination. The method is simple, fast and sensitive, and is very suitable for routine supervision and inspection.