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为了快速纯合杂交后代材料,提高重组基因型的选择几率,对40个杂交F1代组合进行小孢子分离培养。其中,有9个材料产生小孢子胚(占22.5%),仅4个材料(10.0%)获得了再生植株并种植大田,表明基因型是影响小孢子胚胎发生的一个主要因素。4个杂交组合材料的胚胎发生率为0.08~0.86胚/蕾,平均为0.25胚/蕾。细胞学检测表明,本试验杂交组合材料的最适取样花蕾长度为3.5 mm。大田取样采取切取花序,然后在4℃冰箱中插水加散射光保存4~5d不影响胚胎发生。热激培养2 d后小孢子的膨大率是衡量小孢子胚胎能否发生的一个有效指标。
In order to rapidly homozygous hybrid progeny materials and improve the probability of selection of recombinant genotypes, 40 hybrid F1 combinations were isolated and cultured in microspores. Among them, 9 cultivated microspore embryos (22.5%) and only 4 cultivars (10.0%) obtained regenerated plants and planted fields, indicating that genotypes are one of the main factors that affect microspore embryogenesis. Four hybrid combinations of embryo incidence rate of 0.08 ~ 0.86 embryo / bud, an average of 0.25 embryos / bud. Cytological examination showed that the optimal sample length of the hybrid sample was 3.5 mm. Field sampling taken cut inflorescence, and then inserted in the refrigerator at 4 ℃ plus scattered light to save 4 ~ 5d does not affect the embryogenesis. Microspore expansion after 2 days of heat shock culture is an effective measure of microspore embryo development.