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目的 探讨 6号染色体长臂上 8个肿瘤候选基因与非小细胞肺癌发生发展的关系。方法应用多重 PCR对 4 1例非小细胞肺癌中 6号染色体长臂上的 8个肿瘤候选基因的 2 0个微卫星位点进行扩增。 PCR产物应用聚丙烯酰胺凝胶电泳分离 ,电泳结果用 Gene Scan TM,Genotyper TM软件进行分析。结果有 7个基因的 14个微卫星位点杂合性缺失频率超过 2 0 %。分别是 CCNC(M74 0 91,34.5 % ;st SG4 1342 ,10 0 % ) ,FYN (st SG2 9818,2 2 .2 % ;GDB:18710 4 ,38.5 % ) ,IGF2 R (st SG15 19,2 1.1% ;st SG6 2 98,80 % ) ,ML L T4 (N2 6 5 39,2 5 .7% ;sts- AA0 10 818,38.7% ) ,NMBR (st SG6 334,5 0 % ) ,PDCD2 (SGC3135 3,2 7.6 0 % ;sts- N370 94 ,5 0 % ) ,THBS2 (st SG6 890 ,2 5 % ;AA1316 91,2 9.6 0 % ;st SG35 838,4 4 .4 0 % )。结论 这7个肿瘤候选基因可能与非小细胞肺癌发生、发展有关
Objective To explore the relationship between 8 tumor candidate genes on the long arm of chromosome 6 and the development of non-small cell lung cancer. Methods Multiplex PCR was used to amplify 20 microsatellite loci of 8 cancer candidate genes on the long arm of chromosome 6 in 41 cases of non-small cell lung cancer. The PCR products were separated by polyacrylamide gel electrophoresis and the electrophoresis results were analyzed by Gene ScanTM, GenotyperTM software. As a result, the frequency of heterozygosity loss of 14 microsatellite loci in 7 genes exceeded 20%. They are CCNC (M74 0 91, 34.5 %; st SG4 1342, 100%), FYN (st SG2 9818, 22.2%, GDB: 18710 4, 38.5%), IGF2 R (st SG15 19, 2 1.1 %;st SG6 2 98,80 % ),ML L T4 (N2 6 5 39,2 5 .7% ;sts- AA 0 10 818,38.7% ),NMBR (st SG6 334,50% ),PDCD2 (SGC3135 3,2 7.60%; sts-N370 94, 50% ), THBS2 (st SG6 890, 25%; AA1316 91,29.60%; st SG35 838,4 4.4.00%). Conclusion These 7 tumor candidate genes may be related to the occurrence and development of non-small cell lung cancer