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为进一步探讨损伤脊髓侧支出芽的机理，采用聚丙烯酰胺凝胶电泳和改良的悬滴培养法观察电针刺激对备用背根猫脊髓背角组织大于５０ｋｄ组份中神经营养活性物质的影响，以及该物质的生物活性。结果发现：①针刺实验组相对迁移率（ＲＭ）０．１蛋白质含量较对照组明显增加，ＲＭ０．５蛋白质含量明显减少。将其与本室另一非针刺资料相比，ＲＭ０．１蛋白质含量在针刺手术组较非针刺手术组明显增多，而ＲＭ０．５蛋白质含量无显著性变化，表明针刺可促进ＲＭ０．１蛋白质含量增加。②在培养中，随着ＲＭ０．１蛋含量的增多，促进鸡胚背根节神经突起生长的活性也增强，表明此蛋白质具有促进神经突起生长的活性；ＲＭ０．５蛋白质对神经突起的生长没有明显影响。
To further explore the mechanism of spinal cord injury budding injury, polyacrylamide gel electrophoresis and modified hanging drop culture method to observe the electroacupuncture stimulation of spinal cord dorsal horn greater than 50kd component of neurotrophic substances, As well as the biological activity of the substance. The results showed that: (1) The protein content of the relative mobility (RM) 0.1 of the acupuncture experimental group was significantly increased compared with the control group, and the protein content of RM0.5 was significantly decreased. Compared with another non-acupuncture data in this room, the protein content of RM0.1 in the acupuncture group was significantly increased compared with the non-acupuncture group, while the content of RM0.5 protein showed no significant changes, indicating that acupuncture can promote RM0 .1 protein content increased. ②In culture, with the increase of RM0.1 egg content, the activity of promoting neurite outgrowth in chick embryo dorsal root ganglion was also enhanced, indicating that this protein has the activity of promoting the neurite growth; RM0.5 protein has no effect on the neurite growth Clearly affected.