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目的探讨PTEN基因在大肠癌中的表达规律以及在血管形成中的作用。方法采用免疫组化SP法检测72例大肠癌PTEN的蛋白表达以及微血管密度(MVD)。结果大肠癌组织PTEN的表达率明显低于正常结肠黏膜组织(P<0.01)。低分化大肠癌PTEN的表达率显著低于中分化和高分化大肠癌(P<0.01)。PTEN的表达与肿瘤大小、有无淋巴结和肝转移、浸润深度以及Dukes分期无明显关系(P>0.05)。此外,PTEN阳性表达组大肠癌的MVD数量明显低于阴性组(P<0.01)。结论大肠癌中存在PTEN基因的失活,PTEN的表达和大肠癌的分化程度密切相关,并且在肿瘤的血管形成中起一定作用。
Objective To investigate the expression of PTEN gene in colorectal carcinoma and its role in angiogenesis. Methods Immunohistochemical SP method was used to detect the protein expression of PTEN and the microvessel density (MVD) in 72 cases of colorectal cancer. Results The expression of PTEN in colorectal carcinoma was significantly lower than that in normal colonic mucosa (P <0.01). The expression of PTEN in poorly differentiated colorectal cancer was significantly lower than that in moderately differentiated and well-differentiated colorectal cancer (P <0.01). The expression of PTEN had no significant correlation with tumor size, lymph node metastasis, liver metastasis, depth of invasion and Dukes staging (P> 0.05). In addition, the number of MVD in colorectal cancer with PTEN positive expression was significantly lower than that in negative group (P <0.01). Conclusion Inactivation of PTEN gene exists in colorectal cancer. The expression of PTEN is closely related to the differentiation of colorectal cancer and plays a role in the angiogenesis of tumor.