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目的建立肠致病性大肠埃希氏菌(Enteropathogenic E.coli,EPEC)、肠产毒性大肠埃希氏菌(Enterotoxigenic E.coli,ETEC)、肠侵袭性大肠埃希氏菌(Enteroinvasive E.coli,EIEC)、肠黏附性大肠埃希氏菌(Enteroadhesive E.coli,EAEC)、肠出血性大肠埃希氏菌(Enterohmorrhagic E.coli,EHEC)、弥散粘附性大肠埃希氏菌(Diffusely adherent E.coli,DAEC)6种致病性大肠埃希氏菌的傅立叶变换红外光谱(Fourier transform infrared spectroscopy,FT-IR)数据库及FT-IR分型方法。方法应用FT-IR技术对6种致病性大肠埃希氏菌进行指纹图谱数据采集并对其进行基线校正、归一化等处理,应用化学计量学方法对光谱数据进行分析。结果本研究建立了6种致病性大肠埃希氏菌的FT-IR光谱数据库,实现了对6种可疑目标大肠埃希氏菌鉴定;建立了主成分分析(principal component analysis,PCA)和分级聚类分析(hierarchical cluster analysis,HCA)2种模型,成功实现了对6种致病性大肠埃希氏菌的快速分型。结论 FT-IR分析方法快速、简便、易操作,结果重现性好,是一种鉴定6种致病性大肠埃希氏菌的有效方法。
Objective To establish Enteropathogenic E.coli (EPEC), Enterotoxigenic E.coli (ETEC), Enteroinvasive E.coli , EIEC), Enteroadhesive E. coli (EAEC), Enterohaemorrhagic E. coli (EHEC), Diffusely adherent Fourier transform infrared spectroscopy (FT-IR) database and FT-IR typing method for six kinds of pathogenic Escherichia coli (E.coli, DAEC). Methods The fingerprint data of six kinds of pathogenic Escherichia coli were collected by FT-IR technique and corrected by baseline and normalized. The spectral data were analyzed by chemometric method. Results In this study, FT-IR spectra of six pathogenic Escherichia coli strains were established. Six kinds of suspicious target Escherichia coli were identified. Principal component analysis (PCA) and grading Two models of hierarchical cluster analysis (HCA) were successfully established for the rapid typing of six pathogenic Escherichia coli. Conclusion The FT-IR method is rapid, simple and easy to operate with good reproducibility. It is an effective method to identify six kinds of pathogenic Escherichia coli.