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作者采用荧光加吉姆萨(FPG)方法进行姊妹染色单体差别染色,得到令人满意的结果。现以在加有10μM 5-溴脱氧尿嘧啶核苷的培养基中复制了两个周期的中国仓鼠卵巢细胞的中期染色体为例,介绍简化的FPG方法的操作步骤如下: 1.在经空气干燥法制备的载物片上滴两
The authors performed a sister chromatid difference staining using the fluorescence-plus-Giemsa (FPG) method and obtained satisfactory results. For example, the metaphase chromosomes of Chinese Hamster Ovary cells that replicate two cycles in a medium supplemented with 10 μM 5-bromodeoxyuridine are described as follows: 1. After air-drying Law prepared slides on the slide two