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采用正交设计优化紫花苜蓿(Medicagosativa L.)SSR-PCR反应体系,从17对SSR引物中筛选出6对扩增产物具有稳定多态性的引物,检测第1代植株的基因多态性。结果表明:4个紫花苜蓿品种德福(Defi)、德宝(Derby)、阿尔刚金(Algonguin)、三得利(Sanditi)共检测到25个等位基因,每对引物检测出2～8个等位基因,平均为4.17个。结合植株较高、叶色较深、叶片较大、多叶4个表型突变指标,检测经过表型变异筛选的植株等位基因频率及每个位点的多态性信息量(PIC),PIC在0.2216～0.8328之间变化,平均为0.6366。分析多态基因植株与表型变异的相关性,根据检测结果初步确定了13株突变植株,为后继世代遗传变异的多代跟踪及选育奠定基础。
Orthogonal design was used to optimize the SSR-PCR system of alfalfa (Medicago sativa L.). Sixteen SSR primers were screened out from 17 pairs of SSR primers for stable polymorphism of the first generation of plants. The results showed that 25 alleles were detected in 4 alfalfa cultivars, Defi, Derby, Algonguin and Sanditi, with 2 to 8 The average number of genes is 4.17. Combining with the higher plants, darker leaves, larger leaves and more leafy phenotypes, the frequency of alleles and the number of polymorphic information per locus (PIC) PIC varied from 0.2216 to 0.8328 with an average of 0.6366. According to the results of the detection, 13 mutant plants were initially identified, which laid the foundation for the follow-up and breeding of multiple generations of the genetic variation of succeeding generations.