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研究钾水平对人参根系、叶片保护酶活性及膜脂过氧化作用的影响。利用水培的方法,考察0.0 mmol/L,3.0mmol/L,6.0 mmol/L,12.0 mmol/L钾水平对人参根和叶保护酶、活性氧代谢及膜脂过氧化作用的影响。结果表明不同钾水平条件下,保护酶系统对钾水平响应的趋势不同。除叶片中SOD活性一直呈现显著上升趋势外,人参根系保护酶SOD、根叶保护酶POD活性均随着钾水平的升高呈现出先升高后下降的趋势,3.0 mmol/L的条件下活性最强,后期主要表现为被动忍耐。根系SOD、CAT和POD活性小于叶片。CAT活性与SOD和POD趋势不同,呈现出先降低后又升高的趋势,即主要在缺钾和高钾条件下发挥作用,6.0 mmol/L条件下,CAT活性最低。膜脂过氧化产物MDA含量随钾水平的升高先降低之后升高,6.0 mmol/L条件下最低。根系MDA含量小于叶片MDA含量。不同钾水平条件下,SOD、POD、CAT保护酶相互协调作用共同抵御活性氧损伤,促进人参健康生长。
The effects of potassium level on ginseng root, protective enzyme activities and membrane lipid peroxidation were studied. The effects of 0.0 mmol / L, 3.0 mmol / L, 6.0 mmol / L and 12.0 mmol / L potassium on the activities of protective enzymes, active oxygen metabolism and membrane lipid peroxidation in root and leaf of ginseng were investigated by hydroponics. The results showed that under different potassium levels, the tendency of the protective enzyme system to respond to potassium levels varied. In addition to the leaf SOD activity showed a significant upward trend, ginseng root protective enzymes SOD, POD activity of root and leaf protective enzymes with the increase of potassium showed a trend of first increased and then decreased, 3.0 mmol / L under the conditions of the most active Strong, late mainly for passive patience. Root activity of SOD, CAT and POD less than leaves. The activities of CAT and SOD and POD were different, showing a trend of decreasing firstly and then increasing, mainly under the conditions of potassium deficiency and high potassium, and the activity of CAT was the lowest under the condition of 6.0 mmol / L CAT. The MDA content of membrane lipid peroxidation products increased first and then decreased with the increase of K level, but lowest at 6.0 mmol / L. Root MDA content is less than leaf MDA content. Under different potassium levels, SOD, POD and CAT protective enzymes work together to resist reactive oxygen species damage and promote the healthy growth of ginseng.