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目的:观察人酸性成纤维细胞生长因子(haFGF)及其非促分裂型突变体(nmhaFGF)对人脐静脉血管内皮细胞(HEC)一氧化氮(NO)生成的影响。方法:实验分3组,分别为haFGF组、nmhaFGF组和对照组。用Griess反应法检测细胞培养上清NO相对含量(NO2ˉ)。结果:haFGF和nmhaFGF都能诱导内皮细胞NO的生成,但同样条件下,nmhaFGF组诱导产生NO的相对含量与对照组比较在0.10、1.00、10.00、50.00、100.00 ng/mL时均高(P<0.01)。与haFGF组比较在0.10、1.00、10.005、0.00 ng/mL时nmhaFGF组诱导产生NO的相对含量明显下降(P<0.01),在0.01和100.00 ng/mL时也有降低(P<0.05)。结论:nmhaFGF仍保留一定的诱导内皮细胞产生NO的作用,但NO生成量有显著下降。
Objective: To observe the effect of human fibroblast growth factor (haFGF) and its non-mitogenic mutant (nmhaFGF) on nitric oxide (NO) production in human umbilical vein endothelial cells (HEC). Methods: The experiment was divided into 3 groups: haFGF group, nmhaFGF group and control group. Griess reaction was used to detect the relative content of NO in cell culture supernatant (NO2ˉ). Results: Both haFGF and nmhaFGF induced NO production in endothelial cells. However, under the same conditions, the relative levels of NO induced by nmhaFGF were higher than those in control group at 0.10, 1.00, 10.00, 50.00 and 100.00 ng / mL (P < 0.01). Compared with the haFGF group, the relative content of NO induced by nmhaFGF group decreased significantly at 0.10, 1.00, 10.005 and 0.00 ng / mL (P <0.01), and also decreased at 0.01 and 100.00 ng / mL (P <0.05). Conclusion: nmhaFGF still retains a certain role in inducing NO production in endothelial cells, but NO production is significantly reduced.