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目的观察降糖补肾方对小剂量链脲佐菌素加高脂饲料诱导的糖尿病模型大鼠血清TNF-α、IL-6、CRP表达的影响。方法雄性SD大鼠高脂饲料喂养8周后注射小剂量STZ,1周后测大鼠空腹血糖,血糖值>11.1 mmol/L者为造模成功的大鼠,将造模成功的大鼠随机分为模型组、中药不同剂量干预组和阿司匹林干预组,另设空白对照组,药物干预4周,测定各组大鼠给药前后血清TNF-α、IL-6、CRP含量。结果降糖补肾方高、中、低剂量治疗组均能明显降低血清TNF-α水平,并与阿司匹林组相比有统计学差异(P<0.05);降糖补肾方高、中剂量治疗组能明显降低血清IL-6水平,并与阿司匹林组效果相当;降糖补肾方高、中、低剂量治疗组均能明显降低血清CRP水平,并与阿司匹林组相比有统计学差异(P<0.01)。结论降糖补肾方能减轻糖尿病大鼠的炎症状态,对糖尿病大鼠IR有一定程度的改善作用,其机制可能与减轻炎症因子的表达有关。
Objective To observe the effect of Jiangtang Bushen Recipe on serum TNF-α, IL-6 and CRP in diabetic rats induced by low-dose streptozotocin plus high fat diet. Methods Male SD rats were fed with high-fat diet for 8 weeks and then injected with low-dose STZ. After one week, fasting blood glucose was measured in rats. Blood glucose> 11.1 mmol / L was used as the successful model rats. Divided into model group, different doses of traditional Chinese medicine intervention group and aspirin intervention group, another set of blank control group, drug intervention for 4 weeks, before and after treatment in rats were measured TNF-α, IL-6, CRP levels. The results showed that Jiangtang Bushen Fang high, medium and low dose treatment group can significantly reduce the level of serum TNF-α, and compared with aspirin group was statistically significant (P <0.05) Significantly lower serum IL-6 levels, and with aspirin group effect is equivalent; Jiangtang Bushen Fang high, medium and low dose treatment group can significantly reduce serum CRP levels, and compared with aspirin group was statistically significant (P <0.01) . Conclusion Jiangtang Bushen Decoction can relieve the inflammatory state of diabetic rats and improve the IR of diabetic rats to a certain extent, the mechanism may be related to the reduction of the expression of inflammatory cytokines.