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用浸渍接种法人工培养的金龟子绿僵菌对东北大黑鳃金龟进行生物测定,运用时间—剂量—死亡率模型对死亡率随时间、剂量的变化进行了拟合。LC50及其95%置信限为3“43×105(1”17×105～6“40×105)conidia/ml(个分生孢子/毫升),在5”0×105、1“0×106、5”0×106和1“0×107conidia/ml处理下的LT50分别为18”14、14“21、10”27和9“20d。传统生物测定分析方法只是单独分析剂量或时间的效应,而该模型同时考虑了时间与剂量的效应,更适用评价昆虫病原真菌对目标害虫的效力,在生物测定中具有实践和理论价值。
Metarhizium anisopliae was cultured by immersion inoculation method, and the death rate was fitted with time and dose by the time-dose-mortality model. The LC50 and its 95% confidence limits were 3 × 43 1 × 10 6 × 10 6 conidia / ml conidia / ml, The LT50 for the ”0 × 106,5“ × 106 and 1 × ”0 × 107conidia / ml treatments were 18“ 14,14 ”21,10“ 27 and 9 ”, respectively. Conventional bioassays The analysis method only analyzes the effects of dose or time separately. The model considers the effect of time and dose, and is more suitable for evaluating the efficacy of insect pathogenic fungi on target pests. The model has practical and theoretical value in bioassays.