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目的:观察补肾益髓(Bu Shen Yi Sui,BSYS)方及其拆方补肾(Bu Shen,BS)和化痰活血(Hua Tan Huo Xue,HTHX)方对实验性自身免疫性脑脊髓炎(Experimental Autoimmune Encephalomyelitis,EAE)小鼠脑和脊髓中脑源性神经营养因子(Brain-derived Neurotrophic Factor,BDNF)与受体Trk B的影响。方法:将EAE造模小鼠于当天和第7天背部皮下注射髓鞘少突胶质细胞糖蛋白(Myelin Oligodendrocyte Glycoprotein,MOG)35-55抗原,并在免疫当天和第2天后腹腔注射百日咳毒素(Pertussis toxin,PTX)。每日对小鼠进行不同药物的灌胃,并于造模第20和40天分别取脑和脊髓,采用实时荧光定量RTPCR(qRT-PCR)与Western Blot法检测BDNF和Trk B的表达。结果:BSYS及其拆方BS方与HTHX方均可明显上调EAE小鼠脑和脊髓BDNF和Trk B mRNA与蛋白的表达,与模型组比较,差异均有统计学意义(P<0.05或P<0.01)。BSYS方作用优于其BS与HTHX方,但差异无统计学意义(P>0.05)。结论:BSYS及其拆方促进轴突修复的作用可能与增强BDNF/Trk B表达有关,BSYS全方更有显著趋势。
Objective: To observe the effects of Bu Shen Yi Sui (BSYS) and Bu Shen (BS) and Hua Tan Huo Xue (HTHX) on experimental autoimmune encephalomyelitis Effect of Brain-derived Neurotrophic Factor (BDNF) and Receptor Trk B in Autoimmune Encephalomyelitis and EAE Mice. Methods: Myelin Oligodendrocyte Glycoprotein (MOG) 35-55 antigen was injected subcutaneously into the back of EAE model mice on day 7 and day 7, and intraperitoneal injection of pertussis toxin (Pertussis toxin, PTX). The mice were administered with different drugs daily. The brain and spinal cord were harvested on the 20th and 40th day respectively. The expressions of BDNF and Trk B were detected by qRT-PCR and Western Blot. Results: The expression of BDNF and Trk B mRNA and protein in the brain and spinal cord of EAE mice was significantly increased by both BSYS and HTHX prescriptions. Compared with the model group, the differences were statistically significant (P <0.05 or P < 0.01). The effect of BSYS was better than that of BS and HTHX, but the difference was not statistically significant (P> 0.05). Conclusion: The effect of BSYS and its disassembly in promoting axon repair may be related to enhancing the expression of BDNF / Trk B, and the trend of BSYS is more obvious.