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通过‘白茶’和‘智仁早茶’在不同铝氟浓度交互处理的水培试验,研究了茶树的根系活力、叶片的游离脯氨酸含量、质膜透性、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物岐化酶活性(SOD)。结果表明:(1)低浓度铝(30mg.L-1)能够增强茶树根系活力和SOD活性,降低叶质膜透性以及POD和CAT酶活性;(2)在氟浓度为4和12mg.L-1时,与对照相比,茶叶的酶活性增强,叶质膜透性和根系活力均低于对照;(3)铝氟交互处理中,在低铝低氟-低铝高氟交互过程中,POD、CAT和SOD活性均呈上升趋势,可以推断这个铝氟比例范围增强茶树对活性氧的清除,但低铝高氟处理时,POD和CAT活性均比对照低;高铝低氟浓度下,游离脯氨酸含量、根系活力、POD、CAT和SOD活性均较单铝90mg.L-1处理低,可能铝与氟产生协同作用;高铝高氟浓度下,茶苗的POD、CAT和SOD活性较对照高,且叶质膜透性和根系活力比对照低,表明茶树受到一定伤害。这些结果说明,铝氟交互作用对茶树生理机制的影响显著优于单铝和单氟处理,且其作用存在一定比例范围,两个品种间并不一致。
The root activity, free proline content, plasma membrane permeability, catalase (CAT) activity of tea tree were studied through hydroponic experiments of ’White Tea’ and ’Zhiren Morning Tea’ Oxidase (POD) and superoxide dismutase activity (SOD). The results showed that: (1) Low concentrations of aluminum (30mg.L-1) could enhance root activity and SOD activity of tea plants, decrease leaf membrane permeability and POD and CAT activities; (2) Under the conditions of fluorine concentration of 4 and 12 mg.L -1, compared with the control, the enzyme activity of tea leaves increased, leaf membrane permeability and root activity were lower than the control; (3) In the interaction between aluminum and fluoride, in the process of low aluminum-low fluoride-low aluminum-fluoride interaction POD, CAT and SOD all showed an upward trend. It can be inferred that the range of aluminum-fluoride ratio enhanced the removal of reactive oxygen species in tea plants. However, the activities of POD and CAT were lower than those of the control when treated with low aluminum and high fluoride. , Free proline content, root activity, POD, CAT and SOD activities were lower than the single aluminum treatment of 90mg.L-1, aluminum and fluoride may produce synergies; high aluminum and fluoride concentrations, tea seedling POD, CAT and SOD activity was higher than the control, and leaf plasma membrane permeability and root activity were lower than the control, indicating that the tea tree suffered some damage. These results indicate that the interaction between Al and F on the physiological mechanism of tea is significantly better than the single aluminum and single fluorine treatment, and its role in a certain proportion of range, the two varieties are not consistent.