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目的 探讨严重急性呼吸综合征(SARS)患者粪便、痰标本中病毒RNA阳性检出率与病程变化的关系。方法 采取一步法逆转录聚合酶链反应(RT-PCR)对不同病程的临床标本进行SARS冠状病毒(SARS-associated coronavirus,SARS-CoV)RNA的扩增和序列测定,并采用趋势X~2检验探讨标本阳性检出率与不同发病时间的关系。结果 被检样品中扩增出的特异性片段,经测序验证为SARS-CoV序列(同源性100％)。在检测的临床标本中,粪便标本阳性检出率最高(21.55％)。趋势X~2检验显示SARS患者粪便和痰标本中病毒阳性检出率均随着发病时间的增长而下降,X~2=12 55和16.408,P=0.0004和P=0.000 05。结论 一步法RT-PCR可以有效检测SARS患者临床标本存在的SARS-CoV;SARS患者粪便、痰标本RT-PCR阳性率随着发病时间的增长而下降。
Objective To investigate the relationship between the positive detection rate of viral RNA in stool and sputum of patients with severe acute respiratory syndrome (SARS) and the course of disease. Methods One-step reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify and sequence the SARS-associated coronavirus (SARS-CoV) RNA in different clinical stages. The trend X 2 test To explore the positive rate of specimens and the relationship between the different onset time. Results The specific fragment amplified from the test samples was sequenced and confirmed as SARS-CoV sequence (100% homology). Among the tested clinical specimens, the positive rate of stool specimens was the highest (21.55%). The trend X ~ 2 test showed that the positive rate of virus in stool and sputum of SARS patients decreased with the increase of the onset time, X ~ 2 = 12 55 and 16.408, P = 0.0004 and P = 0.000 05. Conclusion One-step RT-PCR can effectively detect the presence of SARS-CoV in clinical specimens of patients with SARS. The positive rate of RT-PCR in feces and sputum of SARS patients decreased with the increase of the onset time.