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目的 用两种不同的脑水肿模型 ,探讨广谱蛋白酶抑制剂对脑水肿的影响。方法 采用经皮光化学照射方法和尾静脉注射过氧化物加缺氧方法建立脑水肿模型 ,用重量法和伊文斯蓝方法评定脑水肿 ,并用电镜和TTC染色进行病理学检查。结果 经皮光化学照射可产生脑梗死和脑水肿。一次尾静脉注射过氧化物加缺氧的方法不产生局灶性脑梗死 ,但可诱发脑水肿。其脑组织含水率和单位脑组织 EB含量与正常组相比明显升高 ,P<0 .0 5。尾静脉注射蛋白酶抑制剂后脑组织含水率和伊文斯蓝含量明显降低 ,P<0 .0 5。结论 蛋白酶抑制剂对以上两种方法形成的脑水肿均有抑制作用。本研究提示蛋白酶级联系统在脑水肿过程中的重要作用 ,临床应用蛋白酶抑制剂治疗脑水肿可能有较好前景。
Objective To investigate the effects of broad-spectrum protease inhibitors on cerebral edema using two different models of cerebral edema. Methods The model of cerebral edema was established by percutaneous photochemical irradiation and the tail vein injection of peroxide and hypoxia. Brain edema was assessed by gravimetric method and Evans blue method. Pathological examination was performed by electron microscopy and TTC staining. Results Percutaneous photochemical irradiation can produce cerebral infarction and cerebral edema. A tail vein injection of peroxide plus hypoxia does not produce focal cerebral infarction, but can induce cerebral edema. The brain water content and brain tissue EB content was significantly higher than the normal group, P <0. Brain tissue water content and Evans blue content decreased significantly after the caudal vein injection of protease inhibitor, P <0.05. Conclusions Protease inhibitors inhibit brain edema formed by the above two methods. This study suggests that the protease cascade system plays an important role in the process of cerebral edema, and clinical application of protease inhibitors may have a better prospect of treating cerebral edema.