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目的探讨E-钙黏附蛋白(epithelia-cadherin,E-cadherin)和E盒结合锌指蛋白2(E-box binding zinc finger protein 2,ZEB2)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及预后意义。方法采用免疫组织化学SP法检测E-cadherin蛋白和ZEB2蛋白在144例NSCLC患者肺癌组织(NSCLC组)及52例NSCLC患者癌旁>5cm的正常肺组织(对照组)中的表达情况,应用Cox回归模型分析预后影响因素。结果NSCLC组ZEB2蛋白表达率(78.5%)高于对照组(11.5%),而E-cadherin蛋白表达率(34.0%)低于对照组(100.0%),差异均有统计学意义(P<0.05);NSCLC组ZEB2、E-cadherin蛋白阳性表达率在肿瘤分化程度、TNM分期和淋巴结转移上差异有统计学意义(P<0.05);NSCLC组ZEB2与E-cadherin蛋白表达呈负相关性(r=-0.444,P=0.000);多因素Cox回归分析显示ZEB2、E-cadherin的表达水平可作为NSCLC预后的独立因素。结论 ZEB2在NSCLC中表达上调,致使E-cadherin蛋白表达下调,从而使肿瘤浸润和转移能力增强,最终导致预后不良。
Objective To investigate the expression of E-cadherin (E-cadherin) and E-box binding zinc finger protein 2 (ZEB2) in non-small cell lung cancer ) Expression and prognostic significance. Methods Immunohistochemical SP method was used to detect the expression of E-cadherin protein and ZEB2 protein in lung cancer tissues (NSCLC group) of 144 NSCLC patients and normal lung tissues (control group)> 5 cm of 52 NSCLC patients. Cox Regression model analysis of prognostic factors. Results The expression of ZEB2 protein in NSCLC group was significantly higher than that in control group (78.5% vs 11.5%), while the expression of E-cadherin protein was significantly lower than that in control group (34.0% vs 100.0%, P <0.05) ). The positive rates of ZEB2 and E-cadherin in NSCLC group were significantly different between tumor differentiation, TNM stage and lymph node metastasis (P <0.05). There was a negative correlation between ZEB2 and E-cadherin protein expression in NSCLC (r = -0.444, P = 0.000). Multivariate Cox regression analysis showed that the expression levels of ZEB2 and E-cadherin were independent prognostic factors for NSCLC. Conclusions ZEB2 is upregulated in NSCLC, which leads to the down-regulation of E-cadherin protein expression, which in turn enhances the ability of tumor invasion and metastasis, leading to poor prognosis.