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[目的]探讨VHL基因突变、CXCR4表达和NFκB激活与结肠癌肝转移的关系。[方法]收集2005年3月～2007年12月Ⅱ～Ⅲ期结肠癌患者67例。分别通过聚合酶链反应—单链构象多态性分析VHL基因突变,RT-PCR检测CXCR4表达,凝胶电泳迁移实验检测NFκB活性,随访24个月。[结果]67例结肠癌患者中CXCR4 mRNA高表达为32.84%(22/67);VHL基因突变率为28.36%(19/67);NFκB激活比例为29.85%(20/67)。随访24个月,16例出现肝转移患者,13例出现其它部位的转移和38例无病情进展。16例出现肝转移患者中CXCR4 mRNA高表达、VHL基因突变和NFκB激活分别为15、11和13例,明显高于未出现转移的其他部位转移的患者和无病情进展患者(P<0.01)。[结论]VHL基因突变和CXCR4上调和NFκB激活可能参与结肠癌肝转移的发生。
[Objective] To investigate the relationship between VHL gene mutation, CXCR4 expression and NFκB activation and hepatic metastasis of colon cancer. [Methods] From March 2005 to December 2007, 67 patients with stage Ⅱ ~ Ⅲ colon cancer were collected. VHL gene mutation was analyzed by polymerase chain reaction-single strand conformation polymorphism, CXCR4 expression was detected by RT-PCR, and NFκB activity was detected by gel electrophoresis migration test, followed up for 24 months. [Results] The high expression of CXCR4 mRNA in 67 colon cancer patients was 32.84% (22/67). The mutation rate of VHL gene was 28.36% (19/67) and the ratio of NFκB activation was 29.85% (20/67). Follow-up 24 months, 16 cases of liver metastases, 13 cases of other parts of the transfer and 38 cases of disease progression. The CXCR4 mRNA was highly expressed in 16 patients with liver metastasis, and the VHL gene mutation and NFκB activation were 15, 11 and 13, respectively, which were significantly higher than those in other metastasis - free patients and those without disease progression (P <0.01). [Conclusion] The mutation of VHL, the up-regulation of CXCR4 and the activation of NFκB may be involved in the occurrence of hepatic metastasis in colon cancer.