目的 :探讨O2 浓度变化在儿童急性淋巴细胞性白血病发生发展中的意义。方法 :O2 测定采用高铁细胞色素C还原法 ,并将沸波醇肉豆寇醋酸酯 (PMA)作为O2 产生的刺激剂 ,以测定细胞对外界刺激的反应。 37℃培养后在5 5 0nm处测其吸收值。结果 :2 8例患儿外周血O2 浓度在化疗前高于正常组 ,化疗后低于正常组 ,PMA刺激后O2产生在化疗前无明显变化 ,在化疗后有统计学意义。结论 :化疗前O2 的高浓度以及化疗后O2 的低浓度 ,O2 产生能力不足提示O2 在急淋的发生发展及抗感染过程中起重要作 Objective: To investigate the significance of the changes of O2 concentration in the development of childhood acute lymphoblastic leukemia. Methods: The determination of O2 was performed by high-speed ferric cytochrome C reduction method and the boiling point alcohol myristic acid acetate (PMA) was used as stimulus to O2 to determine the response of cells to external stimuli. After incubation at 37 ° C, the absorbance was measured at 550 nm. Results: Peripheral blood O2 levels in 28 children were higher than those in the normal group before chemotherapy, and were lower than those in the normal group after chemotherapy. There was no significant change in O2 production before chemotherapy after PMA stimulation, which was statistically significant after chemotherapy. Conclusion: The high concentration of O2 before chemotherapy and the low concentration of O2 after chemotherapy, the lack of O2 production ability suggests that O2 plays an important role in the development and anti-infection of acute lymph