本文着重对骨髓活检标本的透射电镜样品制备中的固定与脱钙问题进行了实验研究。经多种方法试验结果表明,标本经5％戊二醛—4％多聚甲醛混合液固定1天、再经2.5％戊二醛—2％多聚甲醛—5.5％EDTA—Na_2液同时固定与脱钙1天所制备的样—品,超薄切片平整,骨髓造血细胞与成骨细胞、骨细胞的微细结构优良,反差良好。由于标本在预固定的基础上,同时进行固定与脱钙,而减轻了脱钙剂对细胞的损伤作用。因骨髓活检标本脱钙目的在于脱去骨基质中的钙盐,以利于制备良好的切片,故在脱钙足够的情况下,应尽量缩短 This article focuses on the bone marrow biopsy specimens prepared by transmission electron microscopy sample preparation and decalcification problems were studied. The results of various methods showed that the specimens were fixed with 5% glutaraldehyde-4% paraformaldehyde for 1 day and then fixed with 2.5% glutaraldehyde-2% paraformaldehyde-5.5% EDTA-Na 2 Sample prepared by decalcification for one day, the ultrathin sections were flat, the bone marrow hematopoietic cells and osteoblasts, fine structure of bone cells excellent, good contrast. As the specimen in the pre-fixed on the basis of both fixed and decalcified, while reducing the decalcification of cell injury. Because bone marrow biopsy specimens decalcification purposes is to remove calcium in bone matrix, in order to facilitate the preparation of good slices, so decalcification enough, should be as short as possible