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目的从亚洲带绦虫(Taenia asiatica)六钩蚴cDNA文库中克隆出亚洲带绦虫六钩蚴Ta 18基因,生物信息学分析预测其编码蛋白的结构和生物学功能。方法利用生物信息网站如美国国家生物技术信息中心(NCBI,http://www.ncbi.nlm.nih.gov/)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY,http://ca.expasy.org/)中生物信息学分析工具,并结合其他分析软件,分析该基因的结构并预测其编码的蛋白质的结构和功能特征。结果成功克隆出Ta 18基因,全长396 bp,编码131个氨基酸,其序列包含完整开放阅读框,推导出的氨基酸序列与GenBank中牛带绦虫18 kD蛋白的同源性最高,一致性达99%,与猪带绦虫一致性达97%;Ta 18蛋白质的理论分子量为14 810.4 Da,等电点为10.05,蛋白理化性质稳定,分析显示Ta 18蛋白质含有1个信号肽和1个纤连蛋白FnⅢ型结构域。结论成功从亚洲带绦虫六钩蚴中克隆出Ta 18基因并分析预测到所编码蛋白的功能特征。
Objective To clone Ta 18 gene of Taenia asiatica from the cDNA library of Taenia asiatica and to predict its structure and biological function by bioinformatics analysis. Methods Bioinformatics websites such as the National Center for Biotechnology Information (NCBI, http://www.ncbi.nlm.nih.gov/) and the Protein Analysis Expert System of the Swiss Institute of Bioinformatics (ExPASY, http: // ca .expasy.org /) bioinformatics analysis tools, combined with other analysis software to analyze the structure of the gene and predict the structure and function of the protein encoded by it. Results The Ta 18 gene was successfully cloned and contained 396 bp in length and encoded a polypeptide of 131 amino acids. The deduced amino acid sequence of Ta 18 gene has the highest homology with the 18 kD protein of Taenia saginata in GenBank with a consensus of 99 % And Taenia solium was 97%. The theoretical molecular weight of Ta 18 protein was 14 810.4 Da and the isoelectric point was 10.05. The physical and chemical properties of Ta 18 protein were stable. The analysis showed Ta 18 protein contained one signal peptide and one fibronectin Fn type III domain. Conclusion The Ta 18 gene was successfully cloned from Taenia saginata and the functional characteristics of the encoded protein were analyzed.