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目的研究136株金黄色葡萄球菌肠毒素噬菌体分型,探讨马鞍山市金黄色葡萄球菌分离株的肠毒素产生情况以及该菌在此地区的流行特点。方法运用m in iVIDAS全自动荧光免疫分析仪检测肠毒素,采用常规方法进行噬菌体分型。结果136株金黄色葡萄球菌肠毒素检测中有106株为阳性,总阳性率77.9%;23种噬菌体分型有111株被分成12个型别,有90%是Ⅲ群,有25株用本次噬菌体系统无法分型,分辨率达81.6%。结论该地区产肠毒素的金黄色葡萄球菌占有很高的比率,以Ⅲ群噬菌体为主要流行株。
Objective To study the typing of Staphylococcus aureus enterotoxin phage from 136 strains of Staphylococcus aureus isolates in Maanshan City and to find out the epidemiological characteristics of this strain in this area. Methods The enterotoxins were detected by m in iVIDAS automatic fluorescence immunoassay and the phage typing was performed by the conventional method. Results Of 136 strains of Staphylococcus aureus enterotoxins, 106 were positive, with a total positive rate of 77.9%. 111 strains of 23 phage types were divided into 12 types, 90% were group Ⅲ and 25 were used Secondary phage system can not type, resolution of 81.6%. Conclusion The area of enterotoxigenic Staphylococcus aureus occupy a very high rate, with group Ⅲ phage as the main epidemic strain.