论文部分内容阅读
目的探讨紫心甘薯多糖(PPSP)的体内外抗氧化活性。方法采用OH.、DPPH.和还原力反应体系,检测紫心甘薯多糖的体外抗氧化活性,并与维生素C(VC)进行比较。建立链脲佐菌素(STZ)诱导糖尿病大鼠模型,测定并比较PPSP组与模型组的肝脏和胰腺内丙二醛(MDA)含量、还原型谷胱甘肽(GSH)和总抗氧化(T-AOC)活性。结果体外抗氧化实验表明,随着紫心甘薯多糖浓度增加,其还原力、OH.和DPPH.清除率均有所增加。当PPSP浓度为250μg/ml时,表现出较强的还原力,对OH.和DPPH.的清除率分别为92.2%和60.2%,其中对OH.清除率明显高于VC,对DPPH.清除率接近VC清除水平。按PPSP低、中、高剂量(分别为100、200和400 mg/kg.bw)灌胃,结果表明大鼠肝脏和胰腺组织内的MDA含量都有明显降低,GSH和T-AOC活性都有明显升高。肝脏切片结果显示中剂量多糖能有效减少糖尿病所致的肝脏细胞损伤。结论紫心甘薯多糖具有较强的体内外抗氧化活性。
Objective To investigate the antioxidant activity of purple heart sweet potato polysaccharide (PPSP) in vitro and in vivo. Methods The antioxidant activity of purple sweet potato polysaccharides in vitro was detected by OH, DPPH and reducing reaction system, and compared with vitamin C (VC). To establish a rat model of streptozotocin (STZ) -induced diabetes mellitus, and to determine and compare the levels of malondialdehyde (MDA), reduced glutathione (GSH) and total anti-oxidant (MDA) in liver and pancreas of PPSP group and model group T-AOC) activity. Results In vitro antioxidant experiments showed that with the purple sweet potato polysaccharide concentration increased its reducing power, OH and DPPH clearance rate increased. When the concentration of PPSP was 250μg / ml, it showed a strong reducing power, the clearance rates of OH and DPPH were 92.2% and 60.2%, respectively, of which the clearance rate of OH was significantly higher than that of VC, Near VC clearance level. Low, medium and high doses of PPSP (100, 200 and 400 mg / kg.bw, respectively) were intragastrically administered. The results showed that the content of MDA in liver and pancreas of rats decreased significantly, and the activities of GSH and T-AOC Significantly increased. Liver slice results showed that medium dose polysaccharide can effectively reduce diabetic liver cell injury. Conclusion Purple sweet potato polysaccharide has strong antioxidant activity in vitro and in vivo.