单向在体肠灌流在中药研究中的应用

来源 :中国实验方剂学杂志 | 被引量 : 0次 | 上传用户:jianjia88521
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化合物的溶解性和渗透性在评价候选药物的口服吸收及生物药剂学分类系统占有重要地位,基于中药多成分特点,所提出的中药生物药剂学分类系统更适用于中药成分的研究。谱效相关技术的普及为寻找中药药效物质成分提供了新方法,而以肠吸收谱为切入点,避免了血清谱复杂的体内分布、代谢过程带来的难题,且不易遗漏不吸收的有效组分,更加简便,更适用于复方药效物质基础的研究。单向在体肠灌流模型是公认的研究药物在体吸收的模型,在一定程度上准确模拟了口服药物的在体吸收,联用运用肠系膜取血等技术,可较为真实反映成分吸收入血情况,更加适用于中药生物药剂学分类系统及谱效相关的研究,值得注意的是,单向在体肠灌流实际上反映的是各组分表观吸收,欲进一步了解其吸收机制,还需结合单个药物、成分的单向肠灌流研究及在体吸收研究。在单一成分研究的基础上,再对其多成分环境下的特征和规律进行研究,回归到以中药或复方为整体的研究中去。近年来,单向在体肠灌流在中药研究中应用越来越多。查阅近20年文献,对筛选出用于中药研究的200多篇文献进行分析,结合课题组经验,总结其在中药有效成分、单味药、药对、复方研究中的应用及使用时的注意事项,为其在中药研究中的进一步应用提供参考。 The solubility and permeability of the compounds occupy an important position in the evaluation of oral absorption and bio-pharmacy classification systems of candidate drugs. Based on the characteristics of multi-component Chinese medicines, the proposed biopharmaceutics classification system is more suitable for the study of traditional Chinese medicine components. The popularization of spectrum-related technologies has provided a new way to search for the components of traditional Chinese medicine (TCM) efficacy, while taking the intestinal absorption spectrum as the starting point, it avoids the complex distribution of the serum spectrum and the problems caused by the metabolic process, and is not easy to miss the non-absorption effective Component, more simple and more suitable for the study of the basis of compound pharmacodynamics. One-way in-vivo intestinal perfusion model is recognized as a model for studying drug absorption in vivo, which accurately simulates the in-vivo absorption of oral drugs to some extent and uses mesenteric blood sampling in combination with other techniques, which can more accurately reflect the components absorbed into the bloodstream , More suitable for biopharmaceutical classification system of traditional Chinese medicine and spectrum-related research, it is noteworthy that one-way in vivo intestinal perfusion actually reflects the apparent absorption of each component, to further understand its absorption mechanism, but also a combination of Unidirectional intestinal perfusion study of single drug and ingredient and in vivo absorption study. On the basis of single component research, we study the characteristics and rules under the multi-component environment, and return to the study of traditional Chinese medicine or compound as a whole. In recent years, unidirectional intestinal perfusion in the study of traditional Chinese medicine more and more applications. After reviewing nearly 20 years of literature, we analyzed over 200 articles screened for traditional Chinese medicine research. Based on the experience of the research group, we summarized their applications in traditional Chinese medicine (TCM) active ingredients, single herbs, drug pairs and compound studies and their use For its further application in the study of traditional Chinese medicine to provide a reference.
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