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通过IL-2依赖性生长的TH细胞系(FI_2)微量培养[~3H]TdR参入法及国内尚未报道的小鼠胸腺细胞增殖改良法进行了IL-2活性检测。结果提示:前法敏感性高,重复性好,其测定成功之关键在于细胞良好生长状态,后法不需细胞传代培养,简单易行。
The IL-2 activity was detected by microinfusion of [~ 3H] TdR in IL-2-dependent TH cell line (FI_2) and the mouse thymocyte proliferation-ameliorated method that has not been reported in China. The results suggest that the former method has high sensitivity and good repeatability. The key to the success of this method is the good growth state of the cells. The latter method does not require cell subculture and is simple and easy to operate.