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目的:探讨草乌与贝母在煎煮过程及体外模拟消化环境中生物碱类成分的变化规律,初步阐明草乌配伍贝母毒性作用变化原因。方法:采用酸性染料比色法、改良异羟肟酸铁分光光度法和HPLC法,测定草乌与贝母不同配比合煎液及人工胃、肠液处理后溶液中总生物碱和酯型生物碱、乌头碱类成分的含量,并通过主成分分析(PCA)和聚类分析(HCA)综合考察各配比和处理方法对毒性作用变化情况。结果:通过PCA和HCA的综合考察,两者得到的结果相似,均显示11个样品被分为3组,其中样品1为Ⅰ组,样品2、5、9、10、11为Ⅱ组,样品3、4、6、7、8为Ⅲ组,样品的分类结果可能与草乌生物碱和贝母生物碱含量有关;草乌与贝母在一定的比例下毒性增加,尤其是其合煎剂经胃液处理后毒性进一步增大,而经过肠液处理后毒性有一定的降低。结论:“草乌反贝母”是在一定条件下的配伍禁忌,可能与配伍比例和药物体内释放部位有一定的关系。
OBJECTIVE: To investigate the changes of the alkaloids in the process of decoction of Radix Aconiti Kusnezoffii and Fritillaria thunbergii in simulated digestive environment, and to elucidate the causes of the change of the toxicity of Radix Aconiti kusnezoffii in combination with Fritillaria cirrhosa. Methods: Acid dye method, modified hydroxamate spectrophotometry and HPLC method were used to determine the total alkaloids and ester-type organisms Alkaloids and aconitines, and the changes of toxicity were analyzed by principal component analysis (PCA) and cluster analysis (HCA). Results: Through the comprehensive investigation of PCA and HCA, the two obtained similar results, showing that 11 samples were divided into 3 groups, of which sample 1 was group I, samples 2, 5, 9, 10 and 11 were group II, 3,4,6,7,8 for the group Ⅲ, the classification results may be related to the content of Aconitum alkaloids and Fritillaria alkaloids; Aconitum and Fritillaria increased toxicity at a certain ratio, especially the combined decoction Treated by gastric juice further increased toxicity, and after treatment of intestinal fluid toxicity decreased to some extent. Conclusion: “Aconiti Fritillaria ” is a taboo of incompatibility under certain conditions, and may have a certain relationship with the proportion of compatibility and drug release site.