论文部分内容阅读
目的为了解四川省高原地区喜马拉雅旱獭是否携带小肠结肠炎耶尔森菌及该菌是否和鼠疫耶尔森菌存在共生关系。方法 鼠疫常规监测中捕获的喜马拉雅旱獭,解剖时无菌操作取舌根和回盲肠内容物,置于准备好的10～15 ml改良PBS增菌培养管中,在4℃冰箱中增菌10～20 d后分离培养。结果在德格县采样100份,分离小肠结肠炎耶尔森2株,若尔盖县采样148份,分离小肠结肠炎耶尔森菌1株。结论 四川省高原地区喜马拉雅旱獭携带小肠结肠炎结肠炎耶尔森菌,该菌和鼠疫耶尔森菌可能存在共生关系。
Objective To understand whether Himalayan marmot or Yersinia enterocolitica in the plateau region of Sichuan Province and its symbiosis with Y. pestis exist. Methods The Himalayan Marmot caught in routine surveillance of plague was collected during the aseptic operation by placing the tongue base and the cecum back into the prepared 10-15 ml modified PBS enrichment culture tube and enriching 10-20 d after isolation and culture. Results In Dege County, 100 samples were collected, 2 strains of Yersinia enterocolitica and 2 strains of Yersinia enterocolitica were isolated from 148 samples in Zoige prefecture. Conclusion Himalayan marmot carries Yersinia enterocolitica in the plateau area of Sichuan Province. There may be symbiotic relationship between this bacterium and Yersinia pestis.