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目的:建立黄芪中毛蕊异黄酮-7-O-β-D-葡萄吡喃糖苷含量测定的高效液相色谱法,并测定10批不同来源黄芪商品药材中该成分的含量。方法:色谱柱为Polaris C_(18)柱(4.6mm×250mm,5μm),流动相为甲醇-水(30:70),流速为1.0 mL·min~(-1),柱温为25℃,检测波长为254 nm。结果:毛蕊异黄酮-7-O-β-D-葡萄吡喃糖苷在0.010 6~2.12μg呈良好的线性关系,回归方程为Y=3 035.97X-14.85(r=0.999 9),加样回收率平均为95.8%(n=5,RSD=1.3%)。结论:本法简便、快速、灵敏,重现性好。所测10批不同来源黄芪商品药材中毛蕊异黄酮-7-O-β-D-葡萄吡喃糖苷含量差异较大。
Objective: To establish a HPLC method for the determination of calycosin-7-O-β-D-pyranoside in Astragalus membranaceus and determine the content of this component in 10 batches of Astragalus membranaceus from different sources. Method: The column was Polaris C 18 column (4.6mm×250mm, 5μm), the mobile phase was methanol-water (30:70), the flow rate was 1.0 mL·min-1, and the column temperature was 25°C. The detection wavelength is 254 nm. RESULTS: There was a good linear relationship between 0.010 6 -2.12 μg of the cardolose isoflavone-7-O-β-D-glucopyranoside and the regression equation was Y=3 035.97X-14.85 (r=0.999 9). The average was 95.8% (n=5, RSD=1.3%). Conclusion: This method is simple, rapid, sensitive and reproducible. The content of calycosin-7-O-β-D-glucopyranoside in the 10 different batches of Astragalus communis medicinal materials tested was quite different.