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为探讨GLP7基因参与植物抗氧化胁迫的作用机制及其生物学功能,以转GLP7基因大豆T2代株系及其受体品种垦丰16为材料,在氧化剂甲基紫精(MV)胁迫条件下,测定植株叶绿素、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的变化情况。结果表明:相对于非转基因大豆,转基因大豆在MV氧化胁迫下MDA含量降低,总叶绿素含量增加,SOD、CAT活性显著提高。初步证明GLP7的过量表达提高了大豆的抗氧化能力,降低了氧化胁迫对大豆幼苗的损伤。
In order to investigate the mechanism and biological function of GLP7 gene involved in antioxidant stress in plants, the transgenic lines of GLP7 gene T2 generation and its receptor Kenfeng 16 were cultured under oxidative stress with methyl viologen (MV) The content of chlorophyll, malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and catalase (CAT) of the plants were measured. The results showed that compared with non-GMO soybean, the content of MDA, the content of total chlorophyll and the activities of SOD and CAT in transgenic soybean increased significantly under MV oxidative stress. Preliminary evidence that overexpression of GLP7 increased soybean antioxidant capacity and reduced the oxidative stress on soybean seedlings.