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在对虾白斑症病毒 (WSSV)病发生期间 ,养殖虾池内的蟹类也往往出现异常甚至死亡 .通过WSSV对三疣梭子蟹 (Portunustrituberculatus)的人工感染证实了其感染性 .三疣梭子蟹在注射感染后第 9天全部死亡 ,注射后第 6天死亡率最高 ;经口感染 ,三疣梭子蟹在感染后第 2 5天全部死亡 ,在 15～ 2 0d死亡率最高 ;在浸洗感染和对照组 30d内无蟹子死亡 .在感染死亡蟹子和发病虾池内病蟹的鳃、上皮组织的细胞核内电镜观察到的病毒粒子 ,其形态特征与在对虾上观察到的一样 .用PCR和单克隆抗体的FAT法在注射感染和投喂感染的蟹子都得到了WSSV感染阳性结果 ,浸浴感染的蟹子和健康蟹子都得到了WSSV感染阴性结果 .蟹类 (三疣梭子蟹 )对WSSV的敏感性较对虾低 ,为此蟹类有可能是WSSV的携带者或传播者 .
During the period of WSSV, the crabs in farmed shrimp ponds were often abnormal or even dead.The infectivity was confirmed by artificial infection with Portunustrituberculatus by WSSV.After the infection of Portunus trituberculatus All died on the 9th day, and the highest mortality was found on the 6th day after injection. In the case of oral infection, Portunus trituberculatus all died on the 25th day after infection and the highest mortality rate was on the 15th to 20th day. Crab-free death in infected dead crab and pathogens in diseased shrimp crab gills, epithelial cells in the nucleus electron microscopy of the virus particles observed morphological characteristics and observed in shrimp on the same. PCR and monoclonal antibody The results of WSSV infection by FAT method were positive for both infected and fed crabs, and WSSV negative results were obtained for bath-infected crabs and healthy crabs. The sensitivity of crabs (Portunus tritubus) to WSSV Lower than shrimp, for which crabs may be carriers or disseminators of WSSV.