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目的研究蚯蚓提取物地龙2号对实验性大鼠肝纤维化的α肌动蛋白(αSMA)、转化生长因子β1(TGFβ1)、尿激酶型纤溶酶原激活物(uPA)及Ⅰ型纤溶酶原激活抑制物(PAI1)蛋白表达的影响。方法52只雄性Wistar大鼠,随机分成6组:正常组(N);阴性对照组(NC),皮下注射花生油,灌单蒸水;模型组(M),皮下注射CCl4,每日单蒸水灌胃;阳性对照组(PC),造模同时用秋水仙碱0.1mg·kg-1·d-1灌胃;地龙大剂量组(Eh),造模同时用地龙2号50mg·kg-1·d-1灌胃;地龙小剂量组(El),造模同时用地龙2号25mg·kg-1·d-1灌胃。8周末处死大鼠,用免疫组化法检测各组大鼠肝脏组织中αSMA、TGFβ1、uPA及PAI1的表达情况。结果地龙2号大、小剂量组与模型组相比,αSMA、TGFβ1、uPA及PAI1的表达均显著降低,且uPA、PAI1与αSMA及TGFβ1表达下降呈明显的正相关。结论地龙2号可能通过抑制肝纤维化大鼠肝组织中肝星状细胞的活化,降低TGFβ1、uPA及PAI1的表达,发挥抗肝纤维化作用。
Objective To investigate the effects of extract of Chinese wolfberry on the expression of α-actin (αSMA), transforming growth factor β1 (TGFβ1), urokinase-type plasminogen activator (uPA) and type I fibrosis in experimental rat liver fibrosis. The effect of plasminogen activator inhibitor (PAI1) protein expression. Methods Fifty-two male Wistar rats were randomly divided into 6 groups: normal group (N); negative control group (NC) subcutaneously injected with peanut oil and single-dose distilled water; model group (M) subcutaneously injected with CCl4, daily distilled water Gastric gavage; positive control group (PC), intragastric administration with colchicine 0.1 mg·kg-1·d-1; intragastric high-dosage group (Eh), modeling, simultaneous use of Dilong 2 50 mg·kg- 1 d-1 gavage; small doses of earthworms (El), modeling at the same time with Earthworm 2 25mg · kg-1 d-1 gavage. The rats were sacrificed at the end of 8 weeks. The expression of αSMA, TGFβ1, uPA and PAI1 in the liver tissue of each group was detected by immunohistochemistry. Results Compared with the model group, the expression of αSMA, TGFβ1, uPA, and PAI1 were significantly decreased in Dilong 2 large and small dose groups, and there was a significant positive correlation between uPA, PAI1 and the expression of αSMA and TGFβ1. Conclusion Dilong 2 may inhibit the activation of hepatic stellate cells in hepatic tissue of hepatic fibrosis rats, reduce the expression of TGFβ1, uPA and PAI1, and play an anti-hepatic fibrosis effect.