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目的 :评价银杏注射液对枕大池注入 Kaolin诱导的家兔脊髓空洞症的防治作用。方法 :30只中国白兔随机分为 3组。在静脉麻醉下 2 4只动物经皮枕大池穿刺缓慢抽出 0 .6 ml脑脊液后注入等量 2 5 % kaolin;其中 12只于当日起给予银杏注射液静注 5 ml× 7日 ,为银杏组 ;12只以等量生理盐水静注 7天 ,为盐水组 ;6只动物仅作假穿刺和假注射为假手术组以作对照。术后定期行 MRI扫描并取 2周以内灌注的部分组织标本行细胞凋亡、bcl- 2及 bax等研究。结果 :注入 Kaolin后 3～ 16天 ,盐水组有 5只银杏组有 1只死亡。盐水组存活的 7只动物有 2只在 4周、 3只在 6周、 1只在 8周时出现上颈髓或颈胸段空洞 ;银杏组存活的 11只动物中只有 1只在 8周时出现上颈髓空洞。组织切片对比发现同一时间内银杏组组织水肿及神经元变性、坏死等均较盐水组为轻。盐水组神经元凋亡率、Bax反应阳性细胞率高于银杏组 ,而 Bcl- 2反应阳性细胞率低于银杏组。假手术组动物 MRI及组织学观察正常。结论 :银杏注射液可改善Kaolin性肉芽肿引起的上颈髓缺血水肿 ,阻止神经元凋亡
Objective: To evaluate the preventive and therapeutic effects of Ginkgo biloba injection on syringomyelia induced by Kaolin injection in rabbits. Methods: Thirty Chinese white rabbits were randomly divided into three groups. In intravenous anesthesia, 24 animals were slowly perfused with 0. 6 ml cerebrospinal fluid and 25 ml kaolin was injected into the 24 animals. Twelve of them received intravenous injection of Ginkgo biloba 5 ml x 7 days on the same day, ; 12 rats were treated with equal volume of saline intravenously for 7 days for the saline group; 6 animals were only punctured and sham-injected for the sham-operated group. MRI scanning was performed regularly and some of the tissue samples perfused within 2 weeks were assayed for apoptosis, bcl-2 and bax. Results: Three to sixteen days after the injection of Kaolin, one of five ginkgo groups in the saline group died. Seven animals in the saline group survived 2 weeks at 4 weeks, 3 at 6 weeks and 1 at 8 weeks with cavities in the upper cervical or cervical thoracic cavities; only 1 out of 11 animals in the ginkgo group survived at 8 weeks Occur on the cardinal cavity. Tissue section comparison found that the same time, ginkgo tissue edema and neuronal degeneration and necrosis were lighter than the saline group. The apoptotic rate of neurons in the saline group was higher than that of the Ginkgo biloba group, but the rate of Bcl-2 positive cells was lower than that of the Ginkgo biloba group. Sham-operated animals were normal in MRI and histology. Conclusion: Ginkgo biloba injection can improve the ischemic edema caused by Kaolin granuloma and prevent neuronal apoptosis