Through separating and purifying diseased mushrooms infected by Mycogone perniciosa in Gaochun Area,Nanjing City,morphological features of the bacteria were identified,and toxicity of the bacterial strains was tested. The results showed that five bactericides in the experiment had obvious inhibitory effect on the growth of M. perniciosa,especially 50% Prochloraz manganese chloride complex WP 5 g / L had the best efficiency. Through separating and purifying diseased mushrooms infected by Mycogone perniciosa in Gaochun Area, Nanjing City, morphological features of the bacteria were identified, and toxicity of the bacterial strains was tested. The results showed that five bactericides in the experiment had significant inhibitory effect on the growth of M. perniciosa, especially 50% Prochloraz manganese chloride complex WP 5 g / L had the best efficiency.