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目的淫羊藿次苷Ⅱ是淫羊藿中提取分离的单体,为了解淫羊藿次苷Ⅱ对阴茎性功能障碍(ED)的疗效,通过体内试验观察其对阴茎海绵体压力和平均动脉血压的影响及作用机制。方法麻醉下游离大鼠左侧颈总动脉和左侧阴茎海绵体,分别插管与电生理仪连接;游离左侧海绵体神经(CN),采用电生理仪刺激器连接双极电极刺激;检测不同浓度的淫羊藿次苷Ⅱ在刺激CN后对阴茎海绵体压力(ICP)和平均动脉血压(MAP)影响,淫羊藿苷、西地那非、罂粟碱作为对照组。同时观察可溶性环鸟苷酸(sGC)抑制剂H-[1,2,4]噁二唑[4,3-A]喹喔啉(ODQ)、一氧化氮合酶(NOS)抑制剂N~ω硝基-L-精氨酸(LNNA)及一氧化氮生成抑制剂亚甲蓝(methylene blue,MB)对淫羊藿次苷Ⅱ(10~(-4)mol/L)诱发ICP/MAP变化的影响。结果 4组药物均剂量依赖性显著提高ICP(P<0.01),淫羊藿次苷Ⅱ、淫羊藿苷和西地那非对MAP无显著影响(P>0.05),而罂粟碱浓度达10~(-4)mol/L后,即可显著降低MAP(P<0.01)。在受试浓度下,LNNA、MB和ODQ可显著抑制淫羊藿次苷Ⅱ诱发的ICP变化(P<0.01),对西地那非诱发的ICP变化无影响(P>0.05)。结论淫羊藿次苷Ⅱ和淫羊藿苷呈剂量依赖性地提高大鼠阴茎勃起功能(ICP),淫羊藿次苷Ⅱ效价强度为淫羊藿苷的2.44倍,对平均动脉血压没有显著影响,其机制可能与增强阴茎海绵体NO-cGMP通路的活性有关。
Objective Epimedium Ⅱ is extracted from Epimedium, in order to understand the efficacy of Epimedium Ⅱ against penile dysfunction (ED). The effects of Epimedium Ⅱ on penile cavernous pressure and mean arterial pressure Influence of blood pressure and its mechanism. Methods The left common carotid artery and the left corpus cavernosum were ligated into the left common carotid artery under anesthesia. The left corpus cavernosal nerve (CN) was excised by electrophysiological stimulator connected with bipolar electrode. The effects of icariin Ⅱ at different concentrations on intracranial penile pressure (ICP) and mean arterial pressure (MAP) after stimulation of CN, icariin, sildenafil and papaverine were taken as control group. At the same time, we observed the effects of soluble glutamyl acid (sGC) inhibitor H- [1,2,4] oxadiazole [4,3-A] quinoxaline (ODQ), nitric oxide synthase (NOS) The effects of omega-nitro-L-arginine (LNNA) and nitric oxide production inhibitor methylene blue (MB) on ICP / MAP induced by icariside Ⅱ (10 ~ -4 mol / L) The impact of change. Results All the four groups of drugs significantly increased the ICP dose-dependently (P <0.01), but no significant difference was found between the two groups (P> 0.05), but the concentration of papaverine was 10 ~ (-4) mol / L, can significantly reduce the MAP (P <0.01). At the concentrations tested, LNNA, MB and ODQ significantly inhibited the changes of ICP induced by icariside Ⅱ (P <0.01), but had no effect on the changes of ICP induced by sildenafil (P> 0.05). Conclusion Epimedium glycoside Ⅱ and icariin increased the penile erectile function (ICP) in a dose-dependent manner. The potency of icariside Ⅱ was 2.44 times of that of icariin. The mean arterial blood pressure Significantly, its mechanism may be related to the enhancement of the activity of NO-cGMP pathway in the corpus cavernosum.