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目的了解铁蓄积骨质疏松小鼠模型造血干细胞(hematopoietic stem cells,HSCs)指标变化及其与骨代谢指标变化的相关性,探讨骨质疏松范畴“铁蓄积”对造血系统的影响。方法 6~8周C57雄性小鼠分为对照组、实验组,实验组用枸橼酸铁铵(ferric ammonium citrate,FAC)0.1 g/kg干预8周,两组均检测:血清铁蛋白(ferritin,FER)、胶原1型氨基端前肽(procollagen type 1 N-terminal propeptide,P1NP)、破骨标志物(carboxy-terminal telopeptides,CTX)、外周血血常规、股骨远端骨小梁三维形态重建和空间结构参数、HSCs占骨髓细胞比例、HSCs细胞集落形成能力,统计分析两组实验数据。结果实验组骨髓HSCs占骨髓比例4.01%±0.63%较对照组2.61%±0.43%显著升高(P<0.05),实验组HSCs细胞集落形成142个/皿较对照组47个/皿显著增多;实验组血清铁蛋白(13.27±0.72)μg/L较对照组(4.14±0.96)μg/L显著升高,实验组P1NP(4.62±0.76)ng/ml较对照组(8.23±0.66)ng/ml显著降低,实验组CTX(123.54±1.63)ng/ml较对照组(83.36±1.89)ng/ml显著升高(P<0.05);实验组组外周血血小板(1380±103)×109/L较对照组(1023±83)×109/L显著升高(P<0.05);实验组micro-CT检测的骨密度(50.41±10.27)mg/mm~3较对照组(104.68±8.56)mg/mm3显著下降、实验组骨小梁空间结构参数[bone volume/tissue volume,BV/TV;骨体积分数10.18%±1.76%;trabecula thickness,Tb.Th骨小梁厚度(0.057±0.012)mm;trabecula number,Tb.N骨小梁数(1.02±0.21)N/mm]较对照组[BV/TV(19.92%±1.92%);Tb.Th(0.098±0.008)mm;Tb.N(1.45±0.13)N/mm]显著下降(P<0.05)。结论在铁蓄积影响的骨质疏松模型中,铁蓄积不仅对骨代谢指标有显著影响,也对HSCs有显著影响。
Objective To investigate the changes of hematopoietic stem cells (HSCs) index and the relationship with changes of bone metabolism index in the model of osteoporosis rat model of iron accumulation and explore the effect of osteoporosis category “iron accumulation” on hematopoietic system. Methods C57 male mice (6-8 weeks old) were divided into control group, experimental group and experimental group with ferric ammonium citrate (FAC) 0.1 g / kg for 8 weeks. The levels of serum ferritin , FER), procollagen type 1 N-terminal propeptide (P1NP), carboxy-terminal telopeptides (CTX), peripheral blood routine, distal three- And spatial structure parameters, the proportion of HSCs in bone marrow cells, colony forming ability of HSCs, two groups of experimental data were statistically analyzed. Results The proportion of HSCs in the experimental group was significantly higher (4.01% ± 0.63%) than that in the control group (2.61% ± 0.43%) (P <0.05) The level of serum ferritin (13.27 ± 0.72) μg / L in the experimental group was significantly higher than that in the control group (4.14 ± 0.96) μg / L and the level of P1NP in the experimental group was (4.62 ± 0.76) ng / ml (123.54 ± 1.63) ng / ml in the experimental group was significantly higher than that in the control group (83.36 ± 1.89) ng / ml (P <0.05). The level of peripheral blood platelet in the experimental group was (1380 ± 103) × 109 / L The bone density (50.41 ± 10.27) mg / mm ~ 3 in the experimental group was significantly higher than that in the control group (104.68 ± 8.56) mg / mm3 (P <0.05), and the trabecular number (BV / TV) was significantly lower in experimental group than that in trabecula group , Tb.N (1.02 ± 0.21) N / mm] compared with the control group [BV / TV (19.92% ± 1.92%; Tb.Th (0.098 ± 0.008) mm; Tb.N (1.45 ± 0.13) N / mm] significantly decreased (P <0.05). Conclusion In the model of osteoporosis affected by iron accumulation, iron accumulation not only has a significant effect on bone metabolism index, but also has a significant effect on HSCs.