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目的观察塞北紫堇总生物碱(CITA)对异丙肾上腺素(Iso)致大鼠实验性心肌缺血的保护作用,并探讨其可能的作用机制。方法将SD大鼠按体重随机分为6组,分别ig 5、20、100 mg·kg~(-1)CITA,20 mg·kg~(-1)心得安以及等量蒸馏水,每天1次,连续8 d,末次给药30 min后,于腹腔注射350 mg·kg~(-1)水合氯醛麻醉,稳定20 min,记录正常心电图,给予各组大鼠皮下多点注射1 mg·kg~(-1)Iso,对照组则注射等体积的生理盐水,记录注射后20 min内心电图的T波以及J点的变化。试验结束时,经股动脉取血,测定血清中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果与对照组比较,在皮下注射Iso5、20 min时,大鼠心电图的T波明显升高,有统计学意义,而CITA各剂量组均有降低其T波高度的趋势,但作用较弱,与模型组比较,无统计学意义;与对照组比较,心电图J点高度明显降低,有统计学意义,与模型组比较,100 mg CITA可显著对抗Iso降低J点的作用,有统计学意义;与对照组比较,血清中MDA、GSH-Px的含量显著增加,有统计学意义,与对照组比较,其血清中SOD的活性变化不大,无统计学意义。与模型组比较,100、5 mg·kg~(-1)CITA可明显降低Iso大鼠血清中MDA、GSH-Px的水平,有统计学意义。结论 CITA对Iso引起的急性心肌缺血损伤具有保护作用,其作用可能与减弱心肌收缩力、扩张冠状动脉血管、减少自由基生成、阻断过氧化脂质反应有关。
Objective To observe the protective effects of CITP on isoproterenol (Iso) -induced experimental myocardial ischemia in rats and its possible mechanism. Methods SD rats were randomly divided into 6 groups according to body weight: 5, 20, 100 mg · kg -1 CITA, 20 mg · kg -1 propranolol and equal volume of distilled water once a day, The rats were anesthetized by intraperitoneal injection of chloral hydrate (350 mg · kg -1) for 30 min after the last administration for 20 min. The normal electrocardiogram (ECG) was recorded and the rats in each group were injected subcutaneously with 1 mg · kg ~ (-1) Iso. The control group was injected with equal volume of saline, and the changes of T wave and J point of electrocardiogram were recorded within 20 min after injection. At the end of the experiment, blood was taken from the femoral artery and the levels of serum glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Results Compared with the control group, the T wave of the electrocardiogram in rats was significantly increased at 5 min and 20 min after subcutaneous injection, which was statistically significant. However, all the CITA dose groups had the tendency of decreasing the T wave height, but the effect was weak, Compared with the model group, there was no statistical significance. Compared with the control group, the J point height of electrocardiogram significantly decreased, with statistical significance. Compared with the model group, 100 mg CITA could significantly antagonize the effect of Iso on J point reduction with statistical significance; Compared with the control group, the content of MDA and GSH-Px in serum increased significantly, which had statistical significance. Compared with the control group, the activity of SOD in serum did not change much, which was not statistically significant. Compared with the model group, CITA at 100 and 5 mg · kg -1 could significantly reduce the level of MDA and GSH-Px in the serum of Iso rats, which was statistically significant. Conclusion CITA has a protective effect on acute myocardial ischemia induced by Iso, which may be related to reducing myocardial contractility, dilating coronary artery, reducing free radical production and blocking lipid peroxidation reaction.