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以野生大豆江浦野生豆-5为母本,栽培大豆南农06-17为父本杂交所得的316个F2单株及其衍生F2∶3和F2∶4家系为材料,利用JoinMap3.0软件,构建了一张包含210个标记(分子标记207个、形态标记3个),共24个连锁群的大豆分子连锁图谱,覆盖基因组长度2 205.85 cM,标记间平均距离为11.09 cM。利用混合线性模型复合区间作图方法,对2007年F2单株、2008年F2∶3家系及2009年F2∶4家系的全生育期、营养生长期、生殖生长期和生育期结构4个生育期相关性状进行联合世代QTL分析,共检测到15个加性显性QTL和9对上位性QTL;存在QTL共位性(同一标记区间存在不同性状的QTL)以及QTL互作网络(一个QTL可以与多个QTL互作)的现象;贡献率最大的3个QTL为qVP-H-1、qWGP-H-1和qRV-H-1,加性效应解释的遗传变异分别为21.31%、13.14%和9.37%,qWGP-H-1和qVP-H-1的增效等位基因来源于江浦野生豆-5,qRV-H-1的增效等位基因来源于南农06-17。研究结果为生育期性状的分子标记辅助选择、野生大豆优异基因的挖掘及栽培大豆遗传基础的拓宽提供了依据。
316 F2 individuals and their derived F2: 3 and F2: 4 pedigrees derived from the wild soybean Jiangpu Pioneer-5 as the female parent and the cultivated soybean Nannong 06-17 as the male parent were selected as the materials. Using JoinMap 3.0 software, A linkage map of 210 molecular markers (207 molecular markers and 3 morphological markers) with a total of 24 linkage groups was constructed, covering a genome length of 20 205.85 cM with an average distance between markers of 11.09 cM. Using hybrid linear model composite interval mapping method, the growth period of vegetative growth period, reproductive growth period and growth period structure of F2 single plant in 2007, F2: 3 family in 2008 and F2: 4 family in 2009 were studied. A total of 15 additive dominant QTLs and 9 pairs of epistatic QTLs were detected in the QTLs for the related generations. QTLs were co-located (QTLs with different traits in the same marker interval) and QTL networks QTL). The three QTLs with the largest contribution rate were qVP-H-1, qWGP-H-1 and qRV-H-1, and the genetic variations explained by additive effects were 21.31% and 13.14% 9.37%. The synergistic allele of qWGP-H-1 and qVP-H-1 was derived from Jiangpu wild bean-5. The synergistic allele of qRV-H-1 was derived from Nannong 06-17. The results provided the basis for molecular marker-assisted selection of growth traits, excavation of excellent genes in wild soybean and broadening of the genetic basis of cultivated soybean.