Abundant small mafic intrusions occur associated with granitoids along the Gangdisê magmatic belt. In addition to many discrete gabbro bodies within the granitoid plutons, a gabbro-pyroxenite zone occurs along the southern margin of the Gangdisê belt to the north of the Yarlung Zangbo suture. The mafic intrusion zone spatially corresponds to a strong aeromagnetic anomaly, which extends ～1400 km. The mafic intrusions consist of intermittently distributed small bodies and dikes of gabbro and dolerite with accumulates of pyroxenite, olivine pyroxenite, pegmatitic pyroxenite and amphibolite. Much evidence indicates that the Gangdisê gabbro-pyroxenite assemblage is most likely a result of underplating of mantle-derived magma. Detailed field investigation and systematic sampling of the mafic rocks was conducted at six locations along the Lhasa-Xigazê segment of the mafic intrusive zone, and was followed by zircon SHRIMP Ⅱ U-Pb dating. In addition to the ages of two samples previously published (47.0±1 Ma and 48.9±1.1 Ma), the isotopic ages of the remaining four gabbro samples are 51.6±1.3Ma, 52.5±3.0 Ma, 50.2±4.2Ma and 49.9±1.1Ma. The range of these ages (47-52.5 Ma) provide geochronologic constraints on the Eocene timing of magma underplating beneath the Gangdisê belt at ca. 50 Ma. This underplating event post-dated the initiation of the India-Eurasia continental collision by 15 million years and was contemporaneous with a process of magma mixing. The SHRIMP Ⅱ U-Pb isotopic analysis also found several old ages from a few zircon grains, mostly in a range of 479-526 Ma (weighted average age 503± 10 Ma), thus yielding information about the pre-existing lower crust when underplating of mafic magma took place. It is believed that magma underplating was one of the major mechanisms for crustal growth during the Indian-Eurasia collision, possibly corresponding in time to the formation of the 14-16 km-thick "crust-mantle transitional zone"characterized by Vp = 6.85-6.9 km/s.