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目的探讨程序性细胞凋亡因子4(PDCD4)和Ki67蛋白在不同期蕈样肉芽肿(MF)皮损中的表达。方法选择27例MF病变皮肤及5例正常皮肤组织蜡块,分为:红斑/斑块期MF(n=21)、肿瘤期MF(n=6)和正常对照组(n=5),应用免疫组织化学Envison两步法分别检测3组标本表皮及真皮中PDCD4及Ki67的表达。结果①红斑/斑块期MF皮损表皮PDCD4+细胞与正常对照组比较差异无统计学意义(P>0.05),真皮PDCD4+细胞明显少于正常对照组(P<0.05);肿瘤期MF皮损表皮及真皮PDCD4+细胞均少于正常对照组和红斑/斑块期MF(P<0.05);②两组MF表皮Ki67+细胞的数量与正常对照组比较差异无统计学意义(P>0.05),红斑/斑块期MF及肿瘤期MF皮损真皮Ki67+细胞均多于正常对照组(P<0.05);肿瘤期MF皮损真皮Ki67+细胞高于红斑/斑块期MF皮损(P<0.05);③各期MF的表皮及真皮中PDCD4和Ki67的表达无相关性(P>0.05)。结论PDCD4的表达从MF的红斑/斑块期到肿瘤期逐渐减低,真皮中Ki67的表达逐渐增强,MF的发生与发展可能与PDCD4表达降低或缺失有关。
Objective To investigate the expression of programmed cell death factor 4 (PDCD4) and Ki67 in different stages of mycosis fungoides (MF) lesions. Methods 27 cases of MF lesions and 5 cases of normal skin tissue were selected and divided into three groups: erythema / plaque phase MF (n = 21), tumor stage MF (n = 6) and normal control group (n = 5) Immunohistochemistry Envison two-step method were used to detect the expression of PDCD4 and Ki67 in epidermis and dermis of three groups of specimens respectively. Results ① The number of PDCD4 + cells in MF skin lesions at erythema / plaque phase was not significantly different from that in normal control group (P> 0.05), and the percentage of PDCD4 + cells in dermis was significantly lower than that of normal control group (P <0.05) (P <0.05); ② There was no significant difference in the number of Ki67 + cells between MF group and normal control group (P> 0.05), erythema / (P <0.05). The Ki67 + cells in the MF lesions at the tumor stage were higher than the MF lesions at the erythema / plaque stage (P <0.05). ③ There was no correlation between the expression of PDCD4 and Ki67 in the epidermis and dermis of each stage of MF (P> 0.05). Conclusions The expression of PDCD4 gradually decreases from erythema / plaque phase of MF to tumor stage, and the expression of Ki67 in dermis gradually increases. The occurrence and development of MF may be related to the decrease or deletion of PDCD4.