论文部分内容阅读
为了解人胎盘绒毛是否存在运动神经诱向因子１（ＭＮＴＦ１）及其可能的功能意义，本实验用ＭＮＴＦ１单克隆抗体及抗独特型单克隆抗体在人早期胎盘绒毛石蜡切片上进行免疫组织化学反应，对人早期胎盘绒毛的ＭＮＴＦ１及其受体进行定位。结果显示，胎盘绒毛的细胞滋养层细胞、合体滋养层细胞和基质细胞均呈ＭＮＴＦ１强免疫反应，ＭＮＴＦ１样免疫反应物质分布在胞质内，胞核为阴性。在相邻切片上，以上３种细胞同样显示为ＭＮＴＦ１受体免疫反应，ＭＮＴＦ１受体样免疫反应物质也分布在细胞质内，胞核无免疫反应。以上结果显示，人胎盘绒毛可能会产生ＭＮＴＦ１，而且其产生的ＭＮＴＦ１可能通过自分泌和旁分泌方式对胎盘绒毛起局部调节作用。
In order to understand the existence of human placental motility induced by motor nerve factor 1 (MNTF1) and its possible functional significance in this experiment with MNTF1 monoclonal antibody and anti-idiotypic monoclonal antibodies in human early placental paraffin section on the immunohistochemical reaction , Targeting early human placental MNTF1 and its receptors. The results showed that the cytotrophoblast cells, syncytiotrophoblasts and stromal cells of placental villi showed strong MNTF1 immune response, MNTF1-like immunoreactive substances distributed in the cytoplasm, the nucleus was negative. In the adjacent sections, the above three kinds of cells also showed MNTF1 receptor immune response, MNTF1 receptor-like immunoreactive substances are also distributed in the cytoplasm, the nucleus without an immune response. The above results show that human placental hair may produce MNTF1, and its production of MNTF1 may play a role in local regulation of placental villus by autocrine and paracrine methods.