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目的了解食管癌手术中肺动、静脉血液和肝、肺组织IL-6的含量及肺、肝组织IL-6受体(IL-6R)的表达,探讨食管癌手术后高水平IL-6的来源及肺损伤的机理。方法采用ELISA法检测17例食管癌手术患者术前肘静脉血液,手术结束前肺静脉、肺动脉血液、肺组织及肝组织IL-6水平。采用免疫组化法检测手术结束前IL-6R在肺组织和肝组织中的表达。结果术后肺动、静脉血液IL-6水平较术前肘静脉血升高(P均<0.01)。术后肺静脉血IL-6高于肺动脉(P=0.035)。术后肝组织与肺组织之间IL-6水平差异无统计学意义(P=0.57),5例患者肺组织中IL-6R表达,而肝组织无IL-6R表达。结论食管癌手术后患者血液中IL-6水平升高;术后肺内IL-6分布高于全身水平,同时IL-6R在肺组织中表达高于肝组织,提示肺组织本身既可能是IL-6的重要来源,也可能是IL-6的重要靶器官。
Objective To investigate the expression of IL-6 in pulmonary artery and venous blood, liver and lung tissue and the expression of IL-6 receptor (IL-6R) in lung and liver tissues during esophageal cancer operation. Source and mechanism of lung injury. Methods 17 cases of esophageal cancer patients undergoing surgical treatment of elbow venous blood before operation, the level of IL-6 in pulmonary vein, pulmonary artery, lung tissue and liver before operation. Immunohistochemistry was used to detect the expression of IL-6R in lung and liver tissue before surgery. Results The levels of interleukin-6 in pulmonary artery and venous blood were significantly higher than those in elbow vein before operation (all P <0.01). Postoperative pulmonary venous blood IL-6 was higher than pulmonary artery (P = 0.035). There was no significant difference in IL-6 level between the postoperative liver tissue and the lung tissue (P = 0.57). IL-6R expression was detected in 5 lung tissues and no IL-6R in the liver tissue. CONCLUSIONS: IL-6 levels in the blood of patients with esophageal cancer are elevated after operation. The distribution of IL-6 in the lung after operation is higher than that in the whole body. Meanwhile, the expression of IL-6R in the lung tissue is higher than that in the liver tissue, suggesting that the lung tissue itself may be IL -6 is an important source of IL-6 may also be an important target organ.