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【目的】通过对不同地区的宋内志贺菌株进行药物敏感性检测、耐药基因的扩增以及基因分型,了解不同地区宋内志贺菌的耐药情况与流行趋势。【方法】使用微量肉汤稀释法测定了54株宋内志贺菌对21种药物的敏感性,用PCR方法扩增相关耐药基因,利用脉冲场凝胶电泳(Pulsed Field Gel Electrophoresis,PFGE)技术进行宋内志贺菌的基因分型,最后采用Bio Numerics分析软件对所有菌株进行聚类,分析其相似度。【结果】实验菌株对于甲氧苄氨嘧啶/磺胺甲噁唑、四环素、替卡西林/棒酸、氨苄西林、庆大霉素5种抗生素普遍耐药,对亚胺培南、头孢吡肟、左氟沙星、诺氟沙星、阿米卡星5种抗生素全部表现为敏感。共检测出包括bla TEM,bla CTX以及整合子在内的7种不同的耐药基因。全部实验菌株可分为26个不同的PFGE带型,分型后表现出较高的基因同源性。宋内志贺菌的耐药性、携带基因与带型具有一定地域相关性。【结论】目前各地区流行的宋内志贺菌株对甲氧苄氨嘧啶/磺胺甲噁唑与四环素已经普遍耐药,不同地区出现相同聚类分型的菌株表明存在跨区域流行的宋内志贺菌群。因此,加强对不同地区宋内志贺菌的监控对减少多重耐药菌株的产生具有重要意义。
【Objective】 To investigate the drug resistance and epidemiological trend of Shigella sonnei in different areas through drug sensitivity test, amplification of drug resistance genes and genotyping of Shigella strains in different areas. 【Method】 The sensitivity of 54 strains of Shigella sonnei to 21 kinds of drugs was determined by using the broth microdilution method. The genes related to resistance were amplified by PCR. Pulsed Field Gel Electrophoresis (PFGE) Technology for genotyping Shigella sonnei, and finally using Bio Numerics analysis software to cluster all the strains and analyze the similarity. 【Result】 The experimental strains were generally resistant to 5 antibiotics such as trimethoprim / sulfamethoxazole, tetracycline, ticarcillin / clavulanic acid, ampicillin and gentamicin, and were resistant to imipenem, cefepime, Levofloxacin, norfloxacin, amikacin all five antibiotics showed sensitivity. Seven different resistance genes including bla TEM, bla CTX and integron were detected. All the experimental strains can be divided into 26 different PFGE bands, showing high homology after typing. Shigella sonnei resistance, carrying the gene and the band has a certain geographical relevance. 【Conclusion】 Currently, strains of trimethoprim / sulfamethoxazole and tetracycline that are popular in various regions of Shigella flexneri have been generally resistant. Strains of the same cluster in different regions indicate that there exists Song Ci-zhi He bacteria group. Therefore, to strengthen the monitoring of Shigella in different areas of Song multi-resistant strains to reduce the production of great significance.