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介绍了一种将染色体显微操作和PCR技术结合起来进行基因染色体定位的方法 ,具有简便易行 ,特异性和敏感性很高等特点。分析了这种定位方法的技术特点 ,以及SSCP和DNA序列分析等方法在排除错误结果中的运用。
A method of chromosomal micromanipulation combined with PCR technique for gene chromosomal location is introduced, which is characterized by its simplicity, high specificity and high sensitivity. The technical features of this method were analyzed, and the application of SSCP and DNA sequence analysis to eliminate the wrong result was also analyzed.